Li Huawei, Luo Wenbin, Ji Rongchang, Xu Yongqing, Xu Guochun, Qiu Sixin, Tang Hao
Institute of Crop Sciences, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China.
Scientific Observing and Experimental Station of Tuber and Root Crops in South China,Ministry of Agriculture. Fuzhou, Fujian 350013, China.
Heliyon. 2021 Feb 8;7(2):e06002. doi: 10.1016/j.heliyon.2021.e06002. eCollection 2021 Feb.
The potato is an important food crop worldwide. While potatoes are rich in nutrition, the production suffers from yield loss caused by frost and freezing. This study used a common potato cultivar, 'Zhengshu 6', as the study system to measure the changes in the contents of soluble protein, malondialdehyde (MDA), proline, and chlorophyll after 1, 3, 5, and 7 days of low temperature treatment. We performed two-dimensional electrophoresis (2-DE) in combination with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) technology and identified 52 differentially expressed protein spots among these timepoints. Results showed that levels of soluble protein, MDA, and proline increased as the duration of the low temperature treatment increased, and the chlorophyll content decreased. The 52 identified protein spots were classified by function as involved in defense response, energy metabolism, photosynthesis, protein degradation, ribosome formation, signal transduction, cell movement, nitrogen metabolism, and other physiological processes, thus allowing potato plants to achieve metabolic balance at low temperatures.
马铃薯是全球重要的粮食作物。虽然马铃薯营养丰富，但其生产会因霜冻和冰冻导致产量损失。本研究以常见马铃薯品种“郑薯6号”为研究体系，测定低温处理1、3、5和7天后可溶性蛋白、丙二醛（MDA）、脯氨酸和叶绿素含量的变化。我们结合二维电泳（2-DE）和液相色谱-电喷雾电离-串联质谱（LC-ESI-MS/MS）技术，在这些时间点之间鉴定出52个差异表达的蛋白点。结果表明，随着低温处理时间的延长，可溶性蛋白、MDA和脯氨酸水平升高，叶绿素含量降低。鉴定出的52个蛋白点按功能分类，涉及防御反应、能量代谢、光合作用、蛋白质降解、核糖体形成、信号转导、细胞运动、氮代谢和其他生理过程，从而使马铃薯植株在低温下实现代谢平衡。
