College of Agronomy, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Improvement & Germplasm Enhancement, Gansu Agricultural University, Lanzhou, China.
College of Life Science and Technology, Gansu Agricultural University, Lanzhou, China.
Physiol Plant. 2018 May;163(1):103-123. doi: 10.1111/ppl.12670. Epub 2018 Mar 13.
Among the multiple environmental signals and hormonal factors regulating potato tuberization, gibberellins (GAs) are important components of the signaling pathways in these processes. To understand the GAs-signaling response mechanism of potato tuberization, a comparative proteomics approach was applied to analyze proteome change of potato tuberization in vitro subjected to a range of exogenous GA treatments (0, 0.01, 0.1 and 1.0 μM) using two-dimensional gel electrophoresis. Quantitative image analyses showed that a total of 37 protein spots have their abundance significantly altered more than 2-fold. Among these proteins, 13 proteins were up-regulated, 13 proteins were down-regulated, one protein was absent and 10 proteins were induced after treatment by exogenous GA . The MALDI-TOF/TOF MS analyses led to the identification of differentially abundant proteins that are mainly involved in bioenergy and metabolism, storage, signaling, cell defense and rescue, transcription, chaperones, transport. Furthermore, the comparative analysis of GA -responsive proteome allowed for general elucidation of underlying molecular mechanisms of potato tuberization inhibited by exogenous GA . Most of these cellular processes were not conducive to the transition from stolon elongation to tuber formation, including a blockage of starch and storage protein accumulation, the accelerated carbohydrate catabolism, a blockage of JA biosynthesis but an elevated endogenous GAs level, the amplification of GA signal transduction by other signaling pathways, and the regulation of cellular RNA metabolism for controlling tuberization. Our results firstly integrated physiology and proteome data to provide new insights into GA -signaling response mechanisms of potato tuberization in vitro.
在调控马铃薯块茎形成的多种环境信号和激素因子中，赤霉素（GAs）是这些过程中信号通路的重要组成部分。为了了解马铃薯块茎形成过程中 GAs 信号响应机制，采用比较蛋白质组学方法，利用二维凝胶电泳分析了不同外源 GA 处理（0、0.01、0.1 和 1.0 μM）下离体马铃薯块茎化过程中的蛋白质组变化。定量图像分析表明，共有 37 个蛋白质斑点的丰度变化超过 2 倍。这些蛋白质中，13 个蛋白质上调，13 个蛋白质下调，1 个蛋白质缺失，10 个蛋白质在外源 GA 处理后诱导。MALDI-TOF/TOF MS 分析鉴定出差异表达的蛋白质主要涉及生物能和代谢、储存、信号转导、细胞防御和拯救、转录、伴侣蛋白、运输。此外，GA 响应蛋白质组的比较分析阐明了外源 GA 抑制马铃薯块茎形成的潜在分子机制。这些细胞过程大多数不利于从匍匐茎伸长到块茎形成的转变，包括淀粉和贮藏蛋白积累受阻、碳水化合物分解代谢加速、茉莉酸（JA）生物合成受阻但内源 GAs 水平升高、其他信号通路放大 GA 信号转导以及细胞 RNA 代谢的调控以控制块茎形成。我们的研究结果首次将生理学和蛋白质组学数据整合在一起，为体外马铃薯块茎形成过程中的 GA 信号响应机制提供了新的见解。
