Radaeva O A, Simbirtsev A S, Kostina J A
National Research Mordovia State University, Institute of Medicine, Saransk, Russia.
State Research Institute of Highly Pure Biopreparations, St. Petersburg, Russia.
Cytokine X. 2019 Aug 23;1(3):100010. doi: 10.1016/j.cytox.2019.100010. eCollection 2019 Sep.
The purpose of this research is to study the characteristics of the change in the circadian rhythm of macrophage colony-stimulating factor (M-CSF) content in the peripheral blood serum of patients with stage II essential hypertension (EH) based on 5 time points (8:00, 14:00, 20:00, 2:00, and 8:00) and analyze its connection with the frequency of cardiovascular events.
Identified levels of M-CSF in the peripheral blood serum of 60 patients with stage II EH, before and after 1 year of antihypertensive therapy using enzyme-linked immunoassays (at 8:00, 14:00, 20:00, 2:00, and 8:00).
The research demonstrated that stage II EH patients with a medical case history lasting 10-14 years have a greater content of M-CSF in their peripheral blood serum (p > 0.001). Before the start of antihypertensive therapy, they also have an increased variability in the circadian rhythm of M-CSF content in the bloodstream (when compared with healthy individuals) due to an increase at 20:00, decrease at 2:00 and recovery at 8:00. In 70% of those patients taking antihypertensive medication and have reached their target arterial blood pressure, the cytokine decrease stabilizes at 2:00 but the increase at 20:00 remains unchanged. Thirty percent of patients retained the rhythm characteristics of M-CSF content in the blood serum typical of patients before the start of therapy. This is a predictor of an increase in the five-year risk of developing cardiovascular complications, particularly myocardial infarction and acute cerebrovascular accident, in individuals with a comparable risk of cardiovascular complications or death on the Framingham risk score.
本研究的目的是基于5个时间点（8:00、14:00、20:00、2:00和8:00）研究II期原发性高血压（EH）患者外周血血清中巨噬细胞集落刺激因子（M-CSF）含量昼夜节律变化的特征，并分析其与心血管事件发生频率的关系。
采用酶联免疫吸附测定法，在8:00、14:00、20:00、2:00和8:00这5个时间点，测定60例II期EH患者在抗高血压治疗1年前和1年后外周血血清中M-CSF的水平。
研究表明，病史持续10 - 14年的II期EH患者外周血血清中M-CSF含量更高（p>0.001）。在抗高血压治疗开始前，由于20:00时升高、2:00时降低以及8:00时恢复，他们血液中M-CSF含量的昼夜节律变异性也增加（与健康个体相比）。在70%服用抗高血压药物且已达到目标动脉血压的患者中，细胞因子在2:00时的降低趋于稳定，但20:00时的升高保持不变。30%的患者保留了治疗开始前患者血清中M-CSF含量的节律特征。在弗雷明汉风险评分中具有相当心血管并发症或死亡风险的个体中，这是发生心血管并发症，尤其是心肌梗死和急性脑血管意外的五年风险增加的预测指标。
