制备具有 PD1 和 TRAIL 的功能性细胞纳米囊泡以增强抗肿瘤反应的方案。
Protocol to prepare functional cellular nanovesicles with PD1 and TRAIL to boost antitumor response.
机构信息
The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, P.R. China.
Mengchao Med-X Center, Fuzhou University, Fuzhou 350116, P.R. China.
出版信息
STAR Protoc. 2021 Feb 8;2(1):100324. doi: 10.1016/j.xpro.2021.100324. eCollection 2021 Mar 19.
Immunotherapy has achieved notable success in tumor treatment, but it is restricted to a small number of patients due to multiple immunosuppressive pathways in the tumor microenvironment. Here, we present a step-by-step protocol to prepare functional cellular nanovesicles from HEK293-FT cells displaying PD1 and TRAIL. TRAIL specifically induces immunogenic cancer cell death to initiate an immune response, and ectogenic PD1 blocks the PD1/PDL1 checkpoint signal to reactivate anergic tumor-specific CD8+ T cells. For complete details on the use and execution of this protocol, please refer to Wu et al. (2020).
免疫疗法在肿瘤治疗中取得了显著的成功，但由于肿瘤微环境中存在多种免疫抑制途径，其应用受到限制。在这里，我们提供了一个从表达 PD1 和 TRAIL 的 HEK293-FT 细胞制备功能性细胞纳米囊泡的分步方案。TRAIL 特异性诱导免疫原性癌细胞死亡以引发免疫反应，外源性 PD1 阻断 PD1/PDL1 检查点信号以重新激活无能的肿瘤特异性 CD8+T 细胞。有关使用和执行此方案的完整详细信息，请参阅 Wu 等人（2020 年）。
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