利用连接功能测定法研究斑马鱼胚胎中的 RNA 调节蛋白。
Tethered Function Assay to Study RNA-Regulatory Proteins in Zebrafish Embryos.
机构信息
Department of Frontier Life Sciences, Faculty of Lifesciences, Kyoto Sangyo University, Kyoto, Japan.
Department of Biology, Graduate School of Science, Kobe University, Kobe, Hyogo, Japan.
出版信息
Methods Mol Biol. 2021;2218:347-354. doi: 10.1007/978-1-0716-0970-5_27.
Many proteins are assumed to mediate post-transcriptional regulation of mRNAs. However, the lack of information about their target mRNAs and functional domains hampers the detailed analysis of their molecular function. Here we describe a method to analyze the post-transcriptional effects of proteins of interest by artificially tethering the protein to a reporter mRNA in zebrafish embryos.
许多蛋白质被认为可以介导 mRNA 的转录后调控。然而，由于缺乏关于其靶 mRNA 和功能结构域的信息，限制了对其分子功能的详细分析。在这里，我们描述了一种通过在斑马鱼胚胎中人为地将蛋白质与报告 mRNA 连接来分析感兴趣的蛋白质的转录后效应的方法。
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