Peters L J, Mason K A, McBride W H
Br J Cancer. 1977 Sep;36(3):386-90. doi: 10.1038/bjc.1977.204.
Mice depleted of T lymphocytes by thymectomy, whole-body irradiation and bone-marrow reconstitution showed a marked increase in susceptibility to the development of lung colonies after i.v. injection of cells of an immunogenic fibrosarcoma. However, a similar increase was observed in unthymectomized, irradiated and reconstituted mice that had recovered their T-cell function, as evidenced by rejection of allogeneic skin grafts. In both thymectomized and unthymectomized mice subjected to whole-body irradiation, the lung-colony-forming efficiency was high 1 day after irradiation, declined to a minimum at 7 days, and thereafter increased again, unless the animals were held in a pathogen-free environment. Reconstitution of T-cell-depleted mice with thymocytes and/or a thymic lobe graft tended to increase further, rather than reduce, lung-colony-forming efficiency. Induction of profound lymphopenia, by irradiation of the whole body except the thorax, did not significantly increase lung colony yields. These studies show that the lung colony assay is not a reliable method of assessing T-cell function in irradiated mice.
通过胸腺切除、全身照射和骨髓重建使T淋巴细胞耗竭的小鼠，在静脉注射免疫原性纤维肉瘤细胞后，对肺集落形成的易感性显著增加。然而，在未进行胸腺切除、经过照射和重建且已恢复T细胞功能的小鼠中也观察到了类似的增加，这可通过对同种异体皮肤移植的排斥反应得到证明。在接受全身照射的胸腺切除和未胸腺切除的小鼠中，照射后1天肺集落形成效率很高，在第7天降至最低，此后再次升高，除非将动物饲养在无病原体环境中。用胸腺细胞和/或胸腺叶移植对T细胞耗竭的小鼠进行重建，往往会进一步提高而不是降低肺集落形成效率。通过照射除胸部以外的全身诱导严重淋巴细胞减少，并未显著增加肺集落产量。这些研究表明，肺集落测定法不是评估受照射小鼠T细胞功能的可靠方法。
