McHenney M A, Baltz R H
Department of Molecular Genetics, Lilly Research Laboratories, Indianapolis, Indiana 46285.
J Bacteriol. 1988 May;170(5):2276-82. doi: 10.1128/jb.170.5.2276-2282.1988.
A segment (hft) of bacteriophage FP43 DNA cloned into plasmid pIJ702 mediated high-frequency transduction of the resulting plasmid (pRHB101) by FP43 in Streptomyces griseofuscus. The transducing particles contained linear concatemers of plasmid DNA. Lysates of FP43 prepared on S. griseofuscus containing pRHB101 also transduced many other Streptomyces species, including several that restrict plaque formation by FP43 and at least two that produce restriction endonucleases that cut pRHB101 DNA. Transduction efficiencies in different species were influenced by the addition of anti-FP43 antiserum to the transduction plates, the temperature for cell growth before transduction, the multiplicity of infection, and the host on which the transducing lysate was prepared. FP43 lysates prepared on S. griseofuscus(pRHB101) also transduced species of Streptoverticillium, Chainia, and Saccharopolyspora.
克隆到质粒pIJ702中的噬菌体FP43 DNA片段（hft）介导了FP43在灰褐链霉菌中对所得质粒（pRHB101）的高频转导。转导颗粒包含质粒DNA的线性多联体。在含有pRHB101的灰褐链霉菌上制备的FP43裂解物也能转导许多其他链霉菌种，包括几种限制FP43噬菌斑形成的菌种以及至少两种产生能切割pRHB101 DNA的限制性内切酶的菌种。向转导平板中添加抗FP43抗血清、转导前细胞生长的温度、感染复数以及制备转导裂解物所用的宿主都会影响不同菌种中的转导效率。在灰褐链霉菌（pRHB101）上制备的FP43裂解物也能转导轮状链霉菌、链霉菌属和糖多孢菌属的菌种。
