Morino T, Takahashi H, Saito H
Mol Gen Genet. 1985;198(2):228-33. doi: 10.1007/BF00383000.
We constructed a plasmid pR4C1 in which a DNA fragment containing the cohesive ends of an actinophage, R4 was inserted into the ClaI site of plasmid pIJ365. The plasmid pR4C1 was packaged efficiently into R4 phage particles in vivo and therefore transferred by transduction. Southern hybridization analysis of DNA from pR4C1-transducing R4 phage particles indicated that the plasmid DNA was encapsidated as head-to-tail concatemers with the cohesive ends in the termini. We designated the pR4C1 plasmid a cosmid, following the termination of Collins and Hohn (1978).
我们构建了质粒pR4C1，其中含有肌动噬菌体R4粘性末端的DNA片段被插入到质粒pIJ365的ClaI位点。质粒pR4C1在体内能有效地包装到R4噬菌体颗粒中，因此可通过转导进行转移。对来自转导pR4C1的R4噬菌体颗粒的DNA进行Southern杂交分析表明，质粒DNA以头尾串联体的形式被包装，粘性末端位于末端。根据Collins和Hohn（1978年）的定义，我们将pR4C1质粒命名为黏粒。
