利用 CRISPR/Cas9 系统生成同源敲除 ALX1 的人胚胎干细胞系（WAe001-A-060）。
Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system.
机构信息
Department of Neurology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
Department of Neurology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
出版信息
Stem Cell Res. 2021 May;53:102309. doi: 10.1016/j.scr.2021.102309. Epub 2021 Mar 24.
Human ALX1 gene (ALX Homeobox 1) is a protein coding gene and gene ontology annotations related to this gene include DNA-binding transcription factor activity and protein heterdimerization activity. It is necessary for survival of forebrain mesenchyme and may be involved in development of cervix. However, the function of the gene has yet to be determined in humans. Here we generated an ALX1 homozygous human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system. The WAe001-A-060 has a normal undifferentiated morphology and karyotype, pluripotency and three germ layers differentiation potential in vivo.
人类 ALX1 基因（ALX Homeobox 1）是一个编码蛋白质的基因，与该基因相关的基因本体论注释包括 DNA 结合转录因子活性和蛋白质异二聚体活性。它对于前脑间质的存活是必需的，并且可能参与了宫颈的发育。然而，该基因在人类中的功能尚未确定。在这里，我们通过 CRISPR/Cas9 系统生成了一个 ALX1 纯合的人胚胎干细胞系（WAe001-A-060）。WAe001-A-060 具有正常的未分化形态和核型、体内多能性和三个胚层分化潜能。
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