Department of Hematology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.
Department of Oncology, The Air Force Hospital of Southern Theater Command, Guangzhou 510062, China.
Stem Cell Res. 2020 Dec;49:102072. doi: 10.1016/j.scr.2020.102072. Epub 2020 Oct 29.
RGS18 is a member of the RGS (Regulators of G-protein signaling) protein family, involved in megakaryopoiesis, megakaryocyte differentiation and chemotaxis. Here, we created a RGS18 knockout cell line from a human embryonic stem cell line by CRISPR/Cas9 mediated gene targeting, to further understand roles of RGS18 in these processes. The cell line maintains stem cell morphology and normal karyotype, and retains expression of pluripotent marker genes and differentiation potential in vivo. The RGS18 cell line will facilitate investigation of the role of RGS18 during multiple cellular processes in human pluripotent stem cell modeled hematopoiesis.
RGS18 是 RGS（G 蛋白信号转导调节剂）蛋白家族的一员，参与巨核细胞生成、巨核细胞分化和趋化作用。在这里，我们通过 CRISPR/Cas9 介导的基因靶向，从人类胚胎干细胞系中创建了一个 RGS18 敲除细胞系，以进一步了解 RGS18 在这些过程中的作用。该细胞系保持干细胞形态和正常核型，并在体内保留多能标记基因的表达和分化潜能。RGS18 细胞系将有助于研究 RGS18 在人类多能干细胞模型造血过程中的多种细胞过程中的作用。
