Liu Shi-Jie, Meng Ming-Yao, Han Shen, Gao Hui, Zhao Yi-Yi, Yang Yang, Lin Zhu-Ying, Yang Li-Rong, Zhu Kai, Han Rui, Huang Wen-Wen, Wang Run-Qing, Yang Li-Li, Wang Wen-Ju, Li Lin, Wang Xiao-Dan, Hou Zong-Liu, Liao Li-Wei, Yang Li
Central Laboratory of Yan'an Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, People's Republic of China.
Yunnan Cell Biology and Clinical Translation Research Center, Kunming, Yunnan, People's Republic of China.
Rejuvenation Res. 2021 Aug;24(4):283-293. doi: 10.1089/rej.2020.2313. Epub 2021 May 6.
Umbilical cord mesenchymal stem cells (UCMSCs) have been identified as a potentially ideal cell type for use in regenerative therapeutic contexts owing to their excellent paracrine secretory abilities and other desirable properties. Previous work has shown that stem cell-derived exosomes can effectively reduce skin aging, but few studies have specifically focused on the role of UCMSC-derived exosomes in this context. In this study, we isolated exosomes derived from UCMSCs grown in a three-dimensional culture system and explored their ability to modulate the photo-aging of HaCaT keratinocytes. Cell viability and proliferation were assessed using CCK8 assay, whereas wound healing and transwell assays were used to assess cell migratory capabilities. UVB irradiation (60 mJ/cm) was used to induce photo-aging of HaCaT cells. TUNEL and SA-β-Gal staining were used to explore HaCaT cell apoptosis and senescence, respectively, whereas real-time quantitative PCR was used to assess the expression of relevant genes at the mRNA level. We found that UCMSC-derived exosomes were able to enhance normal HaCaT cell proliferation and migration while also inhibiting UVB-induced damage to these cells. These exosomes also reduced HaCaT cell apoptosis and senescence, increasing collagen type I expression and reducing matrix metalloproteinase (MMP1) expression in photo-aged HaCaT cells. Together, these findings indicate that UCMSC-derived exosomes have the potential to be used therapeutically to suppress skin aging.
脐带间充质干细胞（UCMSCs）因其出色的旁分泌能力和其他理想特性，已被确定为再生治疗中潜在的理想细胞类型。先前的研究表明，干细胞衍生的外泌体可以有效延缓皮肤衰老，但很少有研究专门关注UCMSC衍生的外泌体在这方面的作用。在本研究中，我们分离了在三维培养系统中生长的UCMSC衍生的外泌体，并探讨了它们调节HaCaT角质形成细胞光老化的能力。使用CCK8法评估细胞活力和增殖，而伤口愈合实验和Transwell实验则用于评估细胞迁移能力。用UVB照射（60 mJ/cm）诱导HaCaT细胞光老化。分别用TUNEL和SA-β-Gal染色检测HaCaT细胞凋亡和衰老，同时用实时定量PCR在mRNA水平评估相关基因的表达。我们发现，UCMSC衍生的外泌体能够增强正常HaCaT细胞的增殖和迁移，同时抑制UVB对这些细胞的损伤。这些外泌体还减少了HaCaT细胞的凋亡和衰老，增加了光老化HaCaT细胞中I型胶原蛋白的表达，并降低了基质金属蛋白酶（MMP1）的表达。总之，这些发现表明，UCMSC衍生的外泌体具有用于治疗抑制皮肤衰老的潜力。
