Suppr超能文献

脐带间充质干细胞衍生的外泌体作为无细胞治疗具有抗氧化和抗病毒作用。

Umbilical Cord Mesenchymal-Stem-Cell-Derived Exosomes Exhibit Anti-Oxidant and Antiviral Effects as Cell-Free Therapies.

机构信息

Institute of Medical Microbiology, Jinan University, Guangzhou 510632, China.

State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Wuhan 430062, China.

出版信息

Viruses. 2023 Oct 15;15(10):2094. doi: 10.3390/v15102094.

Abstract

The oxidative stress induced by the accumulation of reactive oxygen species (ROS) can lead to cell aging and death. Equally, the skeletal muscle usually hosts enteroviral persistent infection in inflammatory muscle diseases. As excellent bioactive products, the exosomes derived from umbilical cord mesenchymal stem cells (ucMSCs) have been proven to be safe and have low immunogenicity with a potential cell-free therapeutic function. Here, exosomes derived from ucMSCs (ucMSC-EXO) were extracted and characterized. In a model of oxidative damage to skin fibroblasts (HSFs) under exposure to HO, ucMSC-EXO had an observable repairing effect for the HSFs suffering from oxidative damage. Furthermore, ucMSC-EXO inhibited mitogen-activated protein kinases (MAPK), c-Jun N-terminal kinase (JNK), and nuclear factor kappa-B (NF-κB) signaling pathways, thereby promoting p21 protein expression while decreasing lamin B1 protein expression, and finally alleviated oxidative stress-induced cell damage and aging. In a model of rhabdomyosarcoma (RD) cells being infected by enterovirus 71 (EV71) and coxsackievirus B3 (CVB3), the ucMSC-EXO enhanced the expression of interferon-stimulated gene 15 (ISG15) and ISG56 to inhibit enteroviral replication, whereafter reducing the virus-induced proinflammatory factor production. This study provides a promising therapeutic strategy for ucMSC-EXO in anti-oxidative stress and antiviral effects, which provides insight into extending the function of ucMSC-EXO in cell-free therapy.

摘要

活性氧(ROS)积累引起的氧化应激可导致细胞衰老和死亡。同样,在炎症性肌肉疾病中,骨骼肌通常存在肠道病毒持续感染。作为优秀的生物活性产物,脐带间充质干细胞(ucMSC)来源的外泌体已被证明具有安全性和低免疫原性,具有潜在的无细胞治疗功能。在这里,提取并表征了来自脐带间充质干细胞(ucMSC-EXO)的外泌体。在 HO 暴露下皮肤成纤维细胞(HSF)氧化损伤模型中,ucMSC-EXO 对氧化损伤的 HSF 具有明显的修复作用。此外,ucMSC-EXO 抑制丝裂原活化蛋白激酶(MAPK)、c-Jun N 端激酶(JNK)和核因子 kappa-B(NF-κB)信号通路,从而促进 p21 蛋白表达,同时降低 lamin B1 蛋白表达,最终减轻氧化应激诱导的细胞损伤和衰老。在肠道病毒 71(EV71)和柯萨奇病毒 B3(CVB3)感染横纹肌肉瘤(RD)细胞模型中,ucMSC-EXO 增强干扰素刺激基因 15(ISG15)和 ISG56 的表达,抑制肠道病毒复制,从而减少病毒诱导的促炎因子产生。本研究为 ucMSC-EXO 在抗氧化应激和抗病毒作用中的治疗策略提供了新的思路,为进一步拓展 ucMSC-EXO 在无细胞治疗中的功能提供了参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e86e/10612094/99ac2a0d279a/viruses-15-02094-g001.jpg

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验