唾液水平作为口腔鳞状细胞癌的生物标志物
Salivary Level as a Biomarker for Oral Squamous Cell Carcinoma.
作者信息
Ueda Sei, Goto Mitsuo, Hashimoto Kengo, Hasegawa Shogo, Imazawa Masahiko, Takahashi Marico, Oh-Iwa Ichiro, Shimozato Kazuo, Nagao Toru, Nomoto Shuji
机构信息
Department of Maxillofacial Surgery, School of Dentistry, Aichi-gakuin University Graduate School of Medicine, Nagoya, Japan.
Department of Surgery, School of Dentistry, Aichi-gakuin University Graduate School of Medicine, Nagoya, Japan.
出版信息
Cancer Genomics Proteomics. 2021 Mar-Apr;18(2):103-112. doi: 10.21873/cgp.20245.
BACKGROUND/AIM: This study investigated the utility of C-C motif chemokine ligand 20 (CCL20) expression in saliva as a biomarker for oral squamous cell carcinoma (OSCC) and also examined the associated microbiome.
MATERIALS AND METHODS
The study group included patients with OSCC or oral potentially malignant disorder (OPMD), and healthy volunteers (HVs). microarray and qRT-PCR were used to compare salivary CCL20 expression levels among groups. Data on CCL20 levels in oral cancer tissues and normal tissues were retrieved from a public database and examined. Furthermore, next-generation sequencing was used to investigate the salivary microbiome.
RESULTS
A significant increase in the expression level of CCL20 was observed in both OSCC tissues and saliva from patients with oral cancer. Fusobacterium was identified as the predominant bacteria in OSCC and correlated with CCL20 expression level. OSCC screening based on salivary CCL20 expression enabled successful differentiation between patients with OSCC and HVs.
CONCLUSION
CCL20 expression may be a useful biomarker for OSCC.
背景/目的：本研究调查了唾液中C-C基序趋化因子配体20（CCL20）表达作为口腔鳞状细胞癌（OSCC）生物标志物的效用，并研究了相关微生物群。
材料与方法
研究组包括OSCC患者、口腔潜在恶性疾病（OPMD）患者和健康志愿者（HV）。采用微阵列和qRT-PCR比较各组唾液中CCL20的表达水平。从公共数据库中检索并分析口腔癌组织和正常组织中CCL20水平的数据。此外，采用二代测序技术研究唾液微生物群。
结果
在OSCC组织和口腔癌患者的唾液中均观察到CCL20表达水平显著升高。梭杆菌被确定为OSCC中的主要细菌，且与CCL20表达水平相关。基于唾液CCL20表达的OSCC筛查能够成功区分OSCC患者和HV。
结论
CCL20表达可能是OSCC的一个有用生物标志物。
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