Wolf J H, Korf J
Department of Biological Psychiatry, University of Groningen, The Netherlands.
J Chromatogr. 1988 Feb 19;436(3):437-45. doi: 10.1016/s0021-9673(00)94603-x.
A method is described to automate the solid phase catalyzed reaction of a carbonyl group with labels containing a bromomethyl group. The acids to be determined must be soluble in an aprotic, non-aqueous reaction medium, such as acetonitrile. Special emphasis is placed on the solubility of the base, which serves as a catalyst. A suspension of potassium carbonate was prepared in acetonitrile. In this way, the effective surface area of the base is drastically enlarged resulting in faster reaction kinetics. The procedure is illustrated with the detection of fatty acids in serum and rat brain tissue with bromomethylmethoxycoumarin as label. The generality of our approach for HPLC determination of carbonyl compounds is discussed.
本文描述了一种使羰基与含溴甲基的标记物发生固相催化反应自动化的方法。待测定的酸必须可溶于非质子非水反应介质中，如乙腈。特别强调了作为催化剂的碱的溶解性。在乙腈中制备碳酸钾悬浮液。通过这种方式，碱的有效表面积大幅增大，从而加快了反应动力学。用溴甲基甲氧基香豆素作为标记物对血清和大鼠脑组织中的脂肪酸进行检测，对该方法进行了说明。讨论了我们用于高效液相色谱法测定羰基化合物方法的通用性。
