Nutrition, Dietetics and Food Sciences, Utah State University, 8700 Old Main Hill, Logan, UT, 84322-8700, USA.
Nutrition, Dietetics and Food Sciences, Utah State University, 8700 Old Main Hill, Logan, UT, 84322-8700, USA.
Biochimie. 2021 Jun;185:43-52. doi: 10.1016/j.biochi.2021.02.010. Epub 2021 Feb 18.
The composition and metabolic activity of the microbiome affect many aspects of health, and there is current interest in dietary constituents that may affect this system. The purpose of this study was to evaluate the effects of a mix of probiotics, a mix of prebiotics and a bioactive protein fraction on the microbiome, when fed to mice alone and in combination at physiologically relevant doses. Mice were fed the total western diet (TWD) supplemented with prebiotics, probiotics, and bioactive proteins individually and in combination for four weeks. Subsequently, effects on the composition of the gut microbiome, gut short-chain fatty acids (SCFAs) concentration, and gut inflammation were measured. Ruminococcus gnavus was increased in mice gut microbiome after feeding prebiotics. Bifidobacterium longum was increased after feeding probiotics. The treatments significantly affected beta-diversity with minor treatment effects on cecal or fecal SCFAs levels, and the treatments did not affect gut inflammation as measured by fecal calprotectin.
肠道微生物组的组成和代谢活性影响着健康的多个方面，人们对可能影响这一系统的饮食成分很感兴趣。本研究旨在评估当以生理相关剂量单独和组合给予小鼠时，益生菌混合物、益生元混合物和生物活性蛋白质部分对微生物组的影响。 小鼠喂食添加了益生元、益生菌和生物活性蛋白的全西方饮食（TWD）4 周。 随后，测量了对肠道微生物组组成、肠道短链脂肪酸（SCFA）浓度和肠道炎症的影响。 喂食益生元后，小鼠肠道微生物组中的 Ruminococcus gnavus 增加。 喂食益生菌后，Bifidobacterium longum 增加。 这些处理方法显著影响了微生物组的β多样性，对盲肠或粪便 SCFA 水平的影响较小，并且这些处理方法并未通过粪便钙卫蛋白测量来影响肠道炎症。
