College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China; National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Changsha 410128, China.
College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China; National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Changsha 410128, China.
Bioresour Technol. 2021 May;328:124845. doi: 10.1016/j.biortech.2021.124845. Epub 2021 Feb 14.
The succession of the fungal community during the co-composting of chicken manure and crop residues and its role in relation to compost maturity was deciphered using Illumina sequencing and FUNGuild (Fungi + Functional + Guild) tool. In the maturation phase of composting, the relative abundance of pathogenic and symbiotrophic fungi decreased by 68%-85% and 145%-622%, respectively, as compared to the initial phase, which showed 574%-720% increase in the saprotrophic guild. The pathogenic and saprotrophic fungi abundance was correlated to compost maturity represented by germination index and humic spectroscopic ratio (p < 0.05). Random forest analysis and structural equation modeling elucidated the positive effects of the aforementioned fungal taxa on compost maturity, and these effects were mediated by the micro-environmental variables, such as temperature, NH-N/NO-N ratio and total organic carbon content. Our study outlines the importance of fungal community succession for improving composting performance and efficiency.
采用 Illumina 测序和 FUNGuild（真菌+功能+类群）工具，解析了鸡粪和农作物秸秆共堆肥过程中真菌群落的演替及其与堆肥成熟度的关系。在堆肥的成熟阶段，与初始阶段相比，病原真菌和共生真菌的相对丰度分别下降了 68%-85%和 145%-622%，而腐生真菌类群的相对丰度则分别增加了 574%-720%。病原真菌和腐生真菌的丰度与发芽指数和腐殖质光谱比（p<0.05）等代表堆肥成熟度的参数相关。随机森林分析和结构方程模型阐明了上述真菌类群对堆肥成熟度的积极影响，这些影响是由温度、NH-N/NO-N 比和总有机碳含量等微环境变量介导的。本研究概述了真菌群落演替对提高堆肥性能和效率的重要性。
