Porter School of Environment and Earth Sciences, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, Israel; The Triangle Regional Research and Development Center, Kfar Qari 30075, Israel.
Porter School of Environment and Earth Sciences, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, Israel.
Bioresour Technol. 2021 May;328:124815. doi: 10.1016/j.biortech.2021.124815. Epub 2021 Feb 5.
This work aimed to isolate a series of bacterial strains associated with the green seaweed Ulva sp. and evaluate their capability to manufacture PHA. The effect of the type of supplemented sugars found to be in macroalgae, on the growth and PHA productivity of the strains was studied. Analysis of the 16S rRNA gene sequence of the isolated strains revealed that the PHA-producing bacteria were phylogenetically related to the genus Cobetia, Bacillus, Pseudoaltermonas and Sulfitobacter, which showed high PHA contents among the isolates. The highest PHA content was observed in the case of Cobetia strain, with up to 61% w/w in the presence of mannitol and 12% w/w on Ulva sp. acid hydrolysate as a substrate.
本工作旨在分离与绿藻石莼相关的一系列细菌菌株，并评估它们制造 PHB 的能力。研究了在大型海藻中发现的补充糖的类型对菌株生长和 PHB 生产力的影响。对分离菌株的 16S rRNA 基因序列分析表明，产 PHB 的细菌与科氏杆菌属、芽孢杆菌属、假交替单胞菌属和亚硫酸盐杆菌属在系统发育上具有亲缘关系，这些菌株在分离物中显示出较高的 PHB 含量。在甘露醇存在的情况下，Cobetia 菌株的 PHB 含量最高，可达 61%w/w，而在 Ulva sp. 酸水解物作为底物时，PHB 含量为 12%w/w。
