Department of Clinical Sciences, Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Sanandaj Branch Islamic Azad University, Sanandaj, Iran.
Comp Immunol Microbiol Infect Dis. 2021 Apr;75:101620. doi: 10.1016/j.cimid.2021.101620. Epub 2021 Feb 12.
Rift Valley fever (RVF) is a disease caused by RVF virus (RVFV) which can cause infections in a range of wild and domestic ruminants as well as in humans and characterized by an increased incidence of abortion in ruminants. This study aims to survey the seroprevalence and risk factors of this zoonose among aborted sheep in Kurdistan province, the west of Iran. 182 blood samples were collected from aborted sheep during the past one month under age groups <1, ≥1-3, >3-5 year in four seasons in two groups of border and non-border cities of Kurdistan province. The presence of RVFV-specific Antibodies was investigated by using competitive ELISA. Indirect immunofluorescence assay (IIFA) was used to confirm positive samples, after separation of serum, as well as blood samples were analyzed for description of hematological parameters. Of a total sheep sampled 1.65 % (n = 3) were positive for RVFV antibodies in both test. The results of IIFA were correlated with the ELISA results. All of the positive samples showed leucopenia and had significant relation with seroprevalence of RVF (P < 0.05). The seroprevalence of RVF in the border cities were significantly higher than other group (P < 0.05) Age of sheep and season had no significant effect on prevalence of RVF (P > 0.05). Results obtained in this study indicated the presence of low-level RVFV circulation among the sheep of Kurdistan Province in Iran, so it is necessary to carry out further studies in other areas of Iran. Doing an epidemiologically study aimed at isolating RVFV in the ruminants of Kurdistan province is recommended. The risk factor of bordering with Iran's western neighbor (Iraq) requires seriously control of the exchange of animals and the relevant products between the two countries.
裂谷热（RVF）是由裂谷热病毒（RVFV）引起的疾病，可感染多种野生和家养反刍动物以及人类，并以反刍动物流产发病率增加为特征。本研究旨在调查伊朗西部库尔德斯坦省流产绵羊中这种人畜共患病的血清流行率和危险因素。在过去一个月中，根据年龄组<1、≥1-3、>3-5 岁，在库尔德斯坦省的两个边境和非边境城市组中，从流产绵羊中采集了 182 份血液样本。使用竞争性 ELISA 检测 RVFV 特异性抗体的存在。使用间接免疫荧光测定法（IIFA）在分离血清后确认阳性样本，并分析血液样本以描述血液学参数。在总共采集的绵羊中，1.65%（n=3）在两种测试中均对 RVFV 抗体呈阳性。IIFA 的结果与 ELISA 结果相关。所有阳性样本均表现为白细胞减少症，与 RVF 的血清流行率有显著关系（P<0.05）。边境城市的 RVF 血清流行率明显高于其他组（P<0.05）。绵羊的年龄和季节对 RVF 的流行率没有显著影响（P>0.05）。本研究结果表明，伊朗库尔德斯坦省绵羊中存在 RVFV 的低水平循环，因此有必要在伊朗其他地区开展进一步研究。建议在库尔德斯坦省的反刍动物中进行旨在分离 RVFV 的流行病学研究。与伊朗西部邻国（伊拉克）接壤的危险因素需要认真控制两国之间动物和相关产品的交流。
