Giolli R A, Torigoe Y, Blanks R H
Department of Anatomy and Neurobiology, California College of Medicine, University of California, Irvine 92717.
J Comp Neurol. 1988 Mar 1;269(1):73-86. doi: 10.1002/cne.902690106.
The distribution and density of the nonretinal projections to the rabbit medial terminal accessory optic nucleus (MTN) have been studied after injections of horseradish peroxidase (HRP) into the MTN in seven rabbits, and confirmation for the presence of certain of these projections has been made in the rabbit or rat by utilizing anterograde transport of tritiated leucine or leucine/proline after appropriate injections into cerebral cortical areas and brainstem nuclei. In seven cases studied by the retrograde axonal transport method, HRP-labeled neurons have been identified: (A) In four visual or preoculomotor nuclei in which available autoradiographic brain series have confirmed the presence of projections to the MTN: (1) The nucleus of the optic tract/dorsal terminal accessory optic nucleus, (2) the interstitial nucleus of the superior fasciculus (posterior fibers), (3) the periaqueductal gray (including its supraoculomotor portion), and (4) the medial division of the deep mesencephalic nucleus. (B) Within the ventral lateral geniculate nucleus, from which a projection to the MTN has been confirmed autoradiographically in the rat by other workers. (C) In brainstem nuclei and cerebral cortical areas in which available autoradiographic brain series fail to confirm the presence of afferents to the MTN: (1) The nucleus reticularis pontis, pars oralis and pars caudalis, (2) the intermediate interstitial nucleus of the medial longitudinal fasciculus, (3) the nucleus raphe pontis, and (4) five cerebral cortical areas (the area retrosplenialis granularis dorsalis, the striate area, the parietal area 3, the subicular cortex, and the regio praecentralis granularis). Finally, we report retrograde labeling which, on the basis of published connectional data, we believe to result from the spread to and uptake from axons en passant. The false-positive labeling in this category is likely to result from spread of HRP into ventral tegmental nuclei or tracts adjacent to the MTN. Thus, as a result, in the medulla and pons, labeled neurons are found in the medial, lateral, and superior vestibular nuclei, the medullary reticular formation including the nucleus reticularis gigantocellularis, the lateral reticular nucleus, the nucleus raphe magnus, the spinal nucleus of V, the nucleus gracilis/nucleus cuneatus, the dorsal and ventral divisions of the parabrachial nucleus, the central pontine gray, the nucleus K of Meessen and Olszewski, and the dorsal nucleus of the lateral lemniscus.(ABSTRACT TRUNCATED AT 400 WORDS)
在七只家兔的内侧终末副视核（MTN）中注射辣根过氧化物酶（HRP）后，研究了非视网膜投射至家兔MTN的分布和密度。通过向大脑皮质区域和脑干核团进行适当注射后利用氚标记亮氨酸或亮氨酸/脯氨酸的顺行运输，已在家兔或大鼠中证实了其中某些投射的存在。在用逆行轴突运输方法研究的七个案例中，已鉴定出HRP标记的神经元：（A）在四个视觉或动眼神经前核中，现有放射自显影脑图谱已证实存在至MTN的投射：（l）视束核/背侧终末副视核，（2）上纵束间质核（后纤维），（3）导水管周围灰质（包括其动眼神经上部分），以及（4）中脑深核内侧部。（B）在腹外侧膝状核内，其他研究者已通过放射自显影在大鼠中证实了从该核至MTN的投射。（C）在脑干核团和大脑皮质区域中，现有放射自显影脑图谱未能证实存在至MTN的传入纤维：（1）脑桥网状核，口部和尾部，（2）内侧纵束中间间质核，（3）脑桥中缝核，以及（4）五个大脑皮质区域（背侧 retrosplenialis granularis区、纹状区、顶叶3区、海马下皮质和中央前颗粒区）。最后，我们报告了逆行标记，根据已发表的连接数据，我们认为这是由于HRP扩散至并从旁轴突摄取所致。此类假阳性标记可能是由于HRP扩散至MTN附近的腹侧被盖核或纤维束所致。因此，结果是，在延髓和脑桥中，在内侧、外侧和上前庭核、包括巨细胞网状核的延髓网状结构、外侧网状核、中缝大核、三叉神经脊束核、薄束核/楔束核、臂旁核背侧和腹侧部、脑桥中央灰质、Meessen和Olszewski的K核以及外侧丘系背核中发现了标记神经元。（摘要截于400字）
