Dai Hongyu, Wang Yajuan, Ji Shujuan, Kong Ximan, Zhang Fan, Zhou Xin, Zhou Qian
College of Food, Shenyang Agricultural University, Shenyang, China.
College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
Front Plant Sci. 2021 Feb 4;11:590928. doi: 10.3389/fpls.2020.590928. eCollection 2020.
The change of lipid metabolism is a key point of blueberry fruit after refrigeration. This study was conducted to evaluate the effects of intermittent warming (IW) of "DuKe" blueberry fruit on its shelf life at 20 ± 0.5°C following 30 days of refrigeration. IW-treated fruit showed higher contents of phosphatidylcholine, linoleic acid, and oleic acid but lower contents of phosphatidic acid and palmitic acid compared to controls. Protective effects on the cell membrane were also reflected as inhibition of the activity of phospholipase D and lipoxygenase. The blueberry fruit showed a lower decay and pitting incidence with higher firmness than control. Interestingly, IW increased C-repeat binding transcription factor gene expression, which can induce the expression of genes related to hypothermia tolerance in plant cells at low temperature. These results indicate that IW can prevent damage to the membrane lipids, which occurs by senescence at a low temperature of blueberry fruit.
脂质代谢的变化是蓝莓果实冷藏后的关键要点。本研究旨在评估“公爵”蓝莓果实在冷藏30天后,进行间歇性升温(IW)处理对其在20±0.5°C下货架期的影响。与对照组相比,IW处理的果实显示出较高的磷脂酰胆碱、亚油酸和油酸含量,但磷脂酸和棕榈酸含量较低。对细胞膜的保护作用还表现为磷脂酶D和脂氧合酶活性受到抑制。蓝莓果实的腐烂和凹陷发生率较低,硬度高于对照组。有趣的是,IW增加了C-重复结合转录因子基因的表达,该基因可在低温下诱导植物细胞中与低温耐受性相关的基因表达。这些结果表明,IW可以防止蓝莓果实因低温衰老而导致的膜脂损伤。