Post-harvest Biology and Storage of Fruits and Vegetables laboratory, Department of Food Science, Shenyang Agricultural University, 110866, China.
College of Life Science, Sun Yat-sen University, 510275, China.
Plant Cell Physiol. 2018 Jan 1;59(1):167-178. doi: 10.1093/pcp/pcx171.
Bell peppers are vulnerable to low temperature (<7°C) and subject to chilling injury (CI). To elucidate the relationship between cell membrane lipid composition and CI, a membrane lipidomic approach was taken. In addition, we performed microstructural analysis and low-field nuclear magnetic resonance to better understand CI. We also monitored primary physiological metabolism parameters to explain lipidomics. Our study indicated that cellular structure damage was more serious at 4°C, mostly represented by damage to the plasmalemma and plastid degradation. Membrane lipidomic data analysis reveals monogalactosyldiacylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidic acid as crucial biomarkers during CI. Furthermore, the significant increase in proline, electrolyte leakage and phospholipase D in chilled fruits also proved that membrane lipid metabolism is involved in the response to low temperature stress. To our knowledge, this study is the first attempt to describe the CI mechanisms in bell peppers based on membrane lipidomics.
甜椒易受低温（<7°C）影响，遭受冷害（CI）。为了阐明细胞膜脂组成与 CI 之间的关系，采用了膜脂组学方法。此外，我们还进行了微观结构分析和低场核磁共振，以更好地理解 CI。我们还监测了主要的生理代谢参数来解释脂质组学。我们的研究表明，4°C 时细胞结构损伤更严重，主要表现为质膜和质体降解损伤。膜脂组学数据分析表明，在冷害过程中，单半乳糖二酰甘油、磷脂酰胆碱、磷脂酰乙醇胺和磷脂酸是关键生物标志物。此外，冷害果实中脯氨酸、电解质渗漏和磷脂酶 D 的显著增加也证明了膜脂代谢参与了低温胁迫的响应。据我们所知，这项研究是首次尝试基于膜脂组学描述甜椒的 CI 机制。
