Wang Yajuan, Ji Shujuan, Dai Hongyu, Kong Ximan, Hao Jia, Wang Siyao, Zhou Xin, Zhao Yingbo, Wei Baodong, Cheng Shunchang, Zhou Qian
College of Food, Shenyang Agricultural University, Shenyang, China.
Front Plant Sci. 2019 Jun 27;10:829. doi: 10.3389/fpls.2019.00829. eCollection 2019.
Low-temperature storage is the primary postharvest method employed to maintain fruit quality and commercial value. However, pitting can develop during refrigeration, especially during the shelf life. In this study, a membrane lipidomic approach was employed to analyze the potential relationship between pitting and membrane lipid metabolism during post-cold-storage shelf life. We also determined the changes in ultrastructure and water distribution by low-field nuclear magnetic resonance (LF-NMR) and assessed the permeability of membrane, membrane lipid peroxidation, proline and malondialdehyde contents, and the activity and gene expression of phospholipase D and lipoxygenase, which are involved in membrane lipid metabolism. The results indicated that the changes in blueberry phospholipids during storage could be caused by cold stress. Furthermore, dehydration is a manifestation of chilling injury. Finally, the significant increase in electrolyte leakage, content of malondialdehyde and proline, and activity of phospholipase D and lipoxygenase in chilled blueberry also indicated that membrane lipid metabolism plays an important role in cold stress response.
低温贮藏是用于保持果实品质和商业价值的主要采后方法。然而，在冷藏期间，尤其是在货架期，果实可能会出现凹陷。在本研究中，采用膜脂质组学方法分析冷藏后货架期凹陷与膜脂代谢之间的潜在关系。我们还通过低场核磁共振（LF-NMR）测定了超微结构和水分分布的变化，并评估了膜通透性、膜脂过氧化、脯氨酸和丙二醛含量，以及参与膜脂代谢的磷脂酶D和脂氧合酶的活性和基因表达。结果表明，贮藏期间蓝莓磷脂的变化可能是由冷胁迫引起的。此外，脱水是冷害的一种表现。最后，冷藏蓝莓中电解质渗漏、丙二醛和脯氨酸含量以及磷脂酶D和脂氧合酶活性的显著增加也表明膜脂代谢在冷胁迫响应中起重要作用。
