Shen Jiawei, Wu Jian, Yang Yang, Wang Pengcheng, Luo Tao, Guo Yanju, Zhao Jun, Dai Wei, Han Ying, Zhu Peiran, Wu Qiuyue, Li Weiwei, Chen Andrew, Xue Chunyan, Xia Xinyi
COVID-19 Research Center, Institute of Laboratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing Clinical College of Southern Medical University, Nanjing, Jiangsu 210002, China.
Department of Ophthalmology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002, China.
Comput Struct Biotechnol J. 2021 Jan 30;19:1063-1071. doi: 10.1016/j.csbj.2021.01.039. eCollection 2021.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which induced mainly the respiratory damage also caused ocular surface symptoms. However, the detailed description of ocular manifestations, severity fluctuations in confirmed COVID-19 adult patients still lacked. We analyzed onset clinical symptoms and duration, ocular symptoms, needs for medication, outcomes in 28 conjunctivitis patients who were extracted from 3198 COVID-19 patients hospitalized in Huoshenshan Hospital and Taikangtongji Hospital, Wuhan, China. The expression levels of ACE2, TMPRSS2, ANPEP, DPP4, NRP1 on fetal and adult ocular surface and mouse lacrimal glands were assessed by single cell seq analysis. Our results indicated that conjunctivitis was a rare and self-limited complication in adults with COVID-19 while the existence of coronavirus receptors on human ocular surface and mouse lacrimal glands indicated the risk of SARS-CoV-2 infection. Our research firstly examined SARS-CoV-2 receptors, including the new discovered one, NRP1, on the fetal ocular surface and in the mouse lacrimal glands.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）主要引起呼吸道损伤，也会导致眼表症状。然而，对于确诊的新冠肺炎成年患者的眼部表现、严重程度波动情况仍缺乏详细描述。我们分析了从中国武汉火神山医院和泰康同济医院收治的3198例新冠肺炎患者中抽取的28例结膜炎患者的起病临床症状及持续时间、眼部症状、用药需求和转归。通过单细胞测序分析评估胎儿和成人眼表及小鼠泪腺中血管紧张素转换酶2（ACE2）、跨膜丝氨酸蛋白酶2（TMPRSS2）、氨肽酶N（ANPEP）、二肽基肽酶4（DPP4）、神经纤毛蛋白1（NRP1）的表达水平。我们的结果表明，结膜炎在新冠肺炎成年患者中是一种罕见的自限性并发症，而人眼表和小鼠泪腺中存在冠状病毒受体表明存在SARS-CoV-2感染风险。我们的研究首次检测了胎儿眼表和小鼠泪腺中的SARS-CoV-2受体，包括新发现的NRP1。
