Department of Nutrition and Health Sciences University of Nebraska-Lincoln Lincoln Nebraska USA.
Department of Computer Science and Engineering University of Nebraska-Lincoln Lincoln Nebraska USA.
J Extracell Vesicles. 2021 Feb;10(4):e12069. doi: 10.1002/jev2.12069. Epub 2021 Feb 12.
Honey has been used as a nutrient, an ointment, and a medicine worldwide for many centuries. Modern research has demonstrated that honey has many medicinal properties, reflected in its anti-microbial, anti-oxidant, and anti-inflammatory bioactivities. Honey is composed of sugars, water and a myriad of minor components, including minerals, vitamins, proteins and polyphenols. Here, we report a new bioactive component‒vesicle-like nanoparticles‒in honey (H-VLNs). These H-VLNs are membrane-bound nano-scale particles that contain lipids, proteins and small-sized RNAs. The presence of plant-originated plasma transmembrane proteins and plasma membrane-associated proteins suggests the potential vesicle-like nature of these particles. H-VLNs impede the formation and activation of the nucleotide-binding domain and leucine-rich repeat related (NLR) family, pyrin domain containing 3 (NLRP3) inflammasome, which is a crucial inflammatory signalling platform in the innate immune system. Intraperitoneal administration of H-VLNs in mice alleviates inflammation and liver damage in the experimentally induced acute liver injury. miR-4057 in H-VLNs was identified in inhibiting NLRP3 inflammasome activation. Together, our studies have identified anti-inflammatory VLNs as a new bioactive agent in honey.
蜂蜜作为一种营养物质、软膏和药物，在全世界已经使用了好几个世纪。现代研究表明，蜂蜜具有许多药用特性，这反映在其抗菌、抗氧化和抗炎的生物活性上。蜂蜜由糖、水和许多微量成分组成，包括矿物质、维生素、蛋白质和多酚。在这里，我们报告了蜂蜜中的一种新的生物活性成分——囊泡样纳米颗粒（H-VLNs）。这些 H-VLNs 是具有膜结构的纳米级颗粒，含有脂质、蛋白质和小尺寸的 RNA。植物来源的质膜跨膜蛋白和质膜相关蛋白的存在表明这些颗粒可能具有囊泡样的性质。H-VLNs 抑制核苷酸结合域和富含亮氨酸重复相关（NLR）家族、吡喃结构域包含 3（NLRP3）炎性小体的形成和激活，NLRP3 炎性小体是先天免疫系统中一个关键的炎症信号平台。在小鼠中腹腔内给予 H-VLNs 可减轻实验性急性肝损伤中的炎症和肝损伤。H-VLNs 中的 miR-4057 被鉴定为抑制 NLRP3 炎性小体激活的物质。总之，我们的研究表明，抗炎性 VLNs 是蜂蜜中的一种新的生物活性物质。
