Wang Ming-Jing, Liu Wei-Yi, Wang Xue-Ying, Li Yu-Meng, Xiao Hai-Yan, Quan Ri-Cheng, Huang Gang, Hu Xiao-Mei
Department of Hematology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China.
Graduate School, China Academy of Chinese Medical Sciences, Beijing, China.
Front Oncol. 2021 Feb 5;10:606928. doi: 10.3389/fonc.2020.606928. eCollection 2020.
Abnormal autophagy is related to the pathogenesis and clinical symptoms of myelodysplastic syndrome (MDS). However, the effect of autophagy-related genes (ARGs) on the prognosis of MDS remains unclear. Here, we examined the expression profile of 108 patients with MDS from the GSE58831 dataset, and identified 22 genes that were significantly associated with overall survival. Among them, seven ARGs were screened and APIs were calculated for all samples based on the expression of the seven ARGs, and then, MDS patients were categorized into high- and low-risk groups based on the median APIs. The overall survival of patients with high-risk scores based on these seven ARGs was shorter than patients with low-risk scores in both the training cohort (P = 2.851e-06) and the validation cohort (P = 9.265e-03). Additionally, API showed an independent prognostic indicator for survival in the training samples [hazard ratio (HR) = 1.322, 95% confidence interval (CI): 1.158-1.51; P < 0.001] and the validation cohort (HR = 1.05, 95% CI: 1-1.1; P < 0.01). The area under the receiver operating characteristic curve (AUROC) of API and IPSS were 43.0137 and 66.0274 in the training cohorts and the AUC of the validation cohorts were 41.5361 and 72.0219. Our data indicate these seven ARGs can predict prognosis in patients with MDS and could guide individualized treatment.
异常自噬与骨髓增生异常综合征（MDS）的发病机制及临床症状相关。然而，自噬相关基因（ARGs）对MDS预后的影响仍不明确。在此，我们检查了来自GSE58831数据集的108例MDS患者的表达谱，并鉴定出22个与总生存期显著相关的基因。其中，筛选出7个ARGs，并根据这7个ARGs的表达计算所有样本的自噬预后指数（APIs），然后，根据APIs中位数将MDS患者分为高风险组和低风险组。在训练队列（P = 2.851e - 06）和验证队列（P = 9.265e - 03）中，基于这7个ARGs的高风险评分患者的总生存期均短于低风险评分患者。此外，在训练样本[风险比（HR）= 1.322，95%置信区间（CI）：1.158 - 1.51；P < 0.001]和验证队列（HR = 1.05，95% CI：1 - 1.1；P < 0.01）中，APIs显示为生存的独立预后指标。在训练队列中，APIs和国际预后评分系统（IPSS）的受试者工作特征曲线下面积（AUROC）分别为43.0137和66.0274，在验证队列中，AUROC分别为41.5361和72.0219。我们的数据表明，这7个ARGs可预测MDS患者的预后，并可指导个体化治疗。
