Courtens J L, Plöen L, Loir M
I.N.R.A. Physiologie de la Reproduction, Nouzilly, Monnaie, France.
J Reprod Fertil. 1988 Mar;82(2):635-43. doi: 10.1530/jrf.0.0820635.
Protamine was specifically demonstrated in spermatids and spermatozoa of the boar by immunoelectron microscopy, using anti-boar or anti-ram protamine antisera, and three different direct or indirect labelling techniques. The two isomers of the protamine could not be labelled separately. The protamine is present in the cytoplasm of elongating spermatids and it enters the nuclei throughout the elongation process after possible storage in the cytoplasm or in the nuclear envelope of spermatids, or both. These findings differ from previous observations in other species.
通过免疫电子显微镜，使用抗公猪或抗公羊鱼精蛋白抗血清以及三种不同的直接或间接标记技术，在公猪的精子细胞和精子中特异性地证实了鱼精蛋白的存在。鱼精蛋白的两种异构体无法分别标记。鱼精蛋白存在于伸长的精子细胞的细胞质中，在可能储存于精子细胞的细胞质或核膜中，或两者中之后，在整个伸长过程中进入细胞核。这些发现与之前在其他物种中的观察结果不同。
