Department of Biological Sciences, Auburn University, Auburn AL 36849, USA.
Laboratory of Growth Regulators, Faculty of Science, Palacký University & Institute of Experimental Botany, The Czech Academy of Sciences, Šlechtitelů 27, CZ-783 71 Olomouc, Czech Republic.
J Exp Bot. 2021 Apr 2;72(8):3294-3306. doi: 10.1093/jxb/erab076.
Cytokinin response factors (CRFs) are transcription factors that are involved in cytokinin (CK) response, as well as being linked to abiotic stress tolerance. In particular, oxidative stress responses are activated by Clade III CRF members, such as AtCRF6. Here we explored the relationships between Clade III CRFs and oxidative stress. Transcriptomic responses to oxidative stress were determined in two Clade III transcription factors, Arabidopsis AtCRF5 and tomato SlCRF5. AtCRF5 was required for regulated expression of >240 genes that are involved in oxidative stress response. Similarly, SlCRF5 was involved in the regulated expression of nearly 420 oxidative stress response genes. Similarities in gene regulation by these Clade III members in response to oxidative stress were observed between Arabidopsis and tomato, as indicated by Gene Ontology term enrichment. CK levels were also changed in response to oxidative stress in both species. These changes were regulated by Clade III CRFs. Taken together, these findings suggest that Clade III CRFs play a role in oxidative stress response as well as having roles in CK signaling.
细胞分裂素响应因子（CRFs）是参与细胞分裂素（CK）响应的转录因子，并且与非生物胁迫耐受性有关。特别是，Clade III CRF 成员（如 AtCRF6）激活氧化应激反应。在这里，我们探讨了 Clade III CRFs 与氧化应激之间的关系。在两种 Clade III 转录因子（拟南芥 AtCRF5 和番茄 SlCRF5）中，确定了转录组对氧化应激的响应。AtCRF5 需要调节 >240 个参与氧化应激反应的基因的表达。类似地，SlCRF5 参与了近 420 个氧化应激反应基因的调节表达。拟南芥和番茄之间观察到这些 Clade III 成员对氧化应激的基因调控具有相似性，这表明基因本体论术语富集。在这两个物种中，CK 水平也会因氧化应激而发生变化。这些变化受 Clade III CRFs 调节。总之，这些发现表明 Clade III CRFs 在氧化应激反应中发挥作用，并且在 CK 信号转导中也具有作用。
