Department of Botany and Microbiology, Faculty of Science, Damanhour University, Damanhour, Egypt.
Department of Physics, Faculty of Science, Damanhour University, Damanhour, Egypt.
Water Environ Res. 2021 Sep;93(9):1600-1607. doi: 10.1002/wer.1542. Epub 2021 Mar 8.
A novel technique for removing metals from water, by converting them into nanoparticles using the culture filtrate of Fusarium solani YMM20, followed by their removal by centrifugation was developed in this study. It is a promising, simple, and eco-friendly process for the bioremediation of wastewater. This method is built on the reduction of metal ions by cell-free fungal filtrate protein(s). The chitosan and chitosan nanoparticles were used as adsorbents for the removal of Cu , Pb , Co , Cd , Ni , and Fe , separately. In addition, fungal filtrate and chitosan nanoparticles loaded with fungal filtrate were used for the biosynthesis of metal nanoparticles of Cu , Pb , Co , Cd , Ni , and Fe , separately for their removal. The fungal filtrate approach has the highest metal removal (%) for most metals. Among the six metal ions, the fungal filtrate, chitosan nanoparticles loaded with fungal filtrate, chitosan, and chitosan nanoparticles showed the highest removal (%) for Pb and the lowest removal (%) for Co . Techniques such as transmission electron microscopy, scanning electron microscopy, and Zeta potential measurements were used to characterize the obtained nanoparticles. The average size of the spherical nanoparticles observed by transmission electron microscopy was from 18.938 ± 2.269 nm to 60.175 ± 4.973 nm.
本研究开发了一种从水中去除金属的新技术，该技术通过使用尖孢镰刀菌 YMM20 的培养滤液将其转化为纳米颗粒，然后通过离心去除。这是一种有前途的、简单的、环保的废水生物修复方法。该方法基于细胞外真菌滤液蛋白还原金属离子。壳聚糖和壳聚糖纳米颗粒分别用作 Cu、Pb、Co、Cd、Ni 和 Fe 的吸附剂，用于去除。此外，还分别使用真菌滤液和负载真菌滤液的壳聚糖纳米颗粒用于生物合成 Cu、Pb、Co、Cd、Ni 和 Fe 的金属纳米颗粒，以去除它们。真菌滤液法对大多数金属的金属去除率（%）最高。在六种金属离子中，真菌滤液、负载真菌滤液的壳聚糖纳米颗粒、壳聚糖和壳聚糖纳米颗粒对 Pb 的去除率最高，对 Co 的去除率最低。透射电子显微镜、扫描电子显微镜和 Zeta 电位测量等技术用于表征所获得的纳米颗粒。透射电子显微镜观察到的球形纳米颗粒的平均尺寸为 18.938±2.269nm 至 60.175±4.973nm。
