State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
State Key Laboratory of Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
J Antimicrob Chemother. 2020 Aug 1;75(8):2093-2100. doi: 10.1093/jac/dkaa143.
To dissect genomic features of IncpRBL16 plasmids from Pseudomonas.
An extensive genomic comparison was applied to all 17 available sequenced IncpRBL16 plasmids, including 8 sequenced in this study and another 2 sequenced in two of our previous studies.
Conserved IncpRBL16 backbone markers repAIncpRBL16 together with its iterons, parB2-parA, che, pil and ter were present in all 17 plasmids. At least 18 regions or sites across IncpRBL16 genomes exhibited major modular differences, including insertion of accessory modules, deletion of backbone regions surrounding insertion sites and substitution of multiple-gene backbone regions. Ten plasmids carried a sole IncpRBL16 replicon, while exogenous acquisition of an auxiliary replicon (located in an accessory module) besides the primary IncpRBL16 replicon was observed in each of the remaining seven plasmids. The 17 IncpRBL16 plasmids carried at least 71 different accessory modules, notably including Tn1403-related regions, Tn7-family transposons, Tn6571-family transposons, integrative and conjugative elements, and integrative and mobilizable elements. There were a total of 40 known resistance genes, which were involved in resistance to 15 categories of antibiotics and heavy metals, notably including blaIMP-9, blaIMP-45, blaVIM-2, blaDIM-2, blaOXA-246, blaPER-1, aphA and armA.
Different IncpRBL16 plasmids contain different profiles of accessory modules and thus diverse collections of resistance genes. To the best of our knowledge, this is the first report of fully sequenced blaOXA-246-carrying (p12939-PER) and blaPER-1-carrying (p12939-PER and pA681-IMP) IncpRBL16 plasmids and also that of 14 novel (first identified in this study) and additionally 31 newly named (first designated in this study, but with previously determined sequences) mobile elements.
剖析假单胞菌中 IncpRBL16 质粒的基因组特征。
对所有 17 个已测序的 IncpRBL16 质粒进行广泛的基因组比较，包括本研究中测序的 8 个质粒和我们之前的两项研究中测序的另外 2 个质粒。
所有 17 个质粒均存在保守的 IncpRBL16 骨架标记物 repAIncpRBL16 及其倒位重复序列、parB2-parA、che、pil 和 ter。IncpRBL16 基因组中至少有 18 个区域或位点表现出主要的模块差异，包括插入辅助模块、插入位点周围骨架区域的缺失以及多基因骨架区域的替换。10 个质粒仅携带一个 IncpRBL16 复制子，而在其余 7 个质粒中，每个质粒都观察到除主要 IncpRBL16 复制子之外还获得了一个辅助复制子（位于辅助模块中）。这 17 个 IncpRBL16 质粒携带至少 71 个不同的辅助模块，其中包括 Tn1403 相关区域、Tn7 家族转座子、Tn6571 家族转座子、整合和共轭元件以及整合和可移动元件。共有 40 个已知的耐药基因，这些基因参与了 15 类抗生素和重金属的耐药性，其中包括 blaIMP-9、blaIMP-45、blaVIM-2、blaDIM-2、blaOXA-246、blaPER-1、aphA 和 armA。
不同的 IncpRBL16 质粒包含不同的辅助模块谱，因此具有不同的耐药基因集。据我们所知，这是首次完全测序 blaOXA-246 携带（p12939-PER）和 blaPER-1 携带（p12939-PER 和 pA681-IMP）的 IncpRBL16 质粒的报告，也是首次报道 14 个新的（首次在本研究中发现）和另外 31 个新命名的（首次在本研究中命名，但具有先前确定的序列）可移动元件。
