Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171/173, 90-924 Lodz, Poland.
Institute of Food Technology and Analysis, Lodz University of Technology, Stefanowskiego 4/10, 90-924 Lodz, Poland.
Food Chem. 2021 Jul 15;350:129218. doi: 10.1016/j.foodchem.2021.129218. Epub 2021 Feb 6.
The aim of this study was to investigate the in vitro antibacterial mechanisms of Aronia melanocarpa, Chaenomeles superba, and Cornus mas leaf extracts towards meat spoilage and pathogenic bacteria. The extracts decreased bacterial viability after 24 h and 48 h of incubation. Acting as prooxidants, the extracts induced intracellular ROS (reactive oxygen species) generation in bacteria cells, with C. mas having the strongest influence. The leaf extracts increased the release of UV intracellular absorbing components, suggesting a reduction in membrane integrity. They also increased the outer-membrane permeability of the Gram-negative bacteria, with C. superba extract being the most active. Following exposure to the leaf extracts, morphological changes in the bacteria were observed, including the formation of aggregates, EPS synthesis, irregular forms, wrinkled cell surfaces, pores in the cell wall, and shriveling of cells. The leaf extracts inhibited DNA synthesis in E. coli cells by suppressing DNA gyrase activity.
本研究旨在探讨黑果腺肋花楸、木瓜和山茱萸叶提取物对肉类腐败菌和病原菌的体外抑菌机制。提取物在孵育 24 小时和 48 小时后降低了细菌的活力。提取物作为促氧化剂，诱导细菌细胞内 ROS（活性氧）的产生，其中山茱萸叶提取物的影响最强。叶提取物增加了 UV 细胞内吸收成分的释放，表明膜完整性降低。它们还增加了革兰氏阴性菌的外膜通透性，其中木瓜叶提取物最活跃。暴露于叶提取物后，观察到细菌形态发生变化，包括形成聚集物、EPS 合成、不规则形态、皱缩的细胞表面、细胞壁上的孔和细胞收缩。叶提取物通过抑制 DNA 拓扑异构酶活性抑制大肠杆菌细胞中的 DNA 合成。
