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(米契尔)埃利奥特、L. 和林德利的叶子提取物的成分及抗菌活性

Composition and Antibacterial Activity of (Michx.) Elliot, L. and Lindl. Leaf Extracts.

作者信息

Efenberger-Szmechtyk Magdalena, Nowak Agnieszka, Czyżowska Agata, Kucharska Alicja Z, Fecka Izabela

机构信息

Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171/173, 90-924 Lodz, Poland.

Department of Fruit, Vegetable and Plant Nutraceutical Technology, Wrocław University of Environmental and Life Science, Chełmońskiego 37, 51-630 Wrocław, Poland.

出版信息

Molecules. 2020 Apr 25;25(9):2011. doi: 10.3390/molecules25092011.

Abstract

The purpose of this study was to investigate the composition of leaf extracts from , , and , and their antimicrobial activity against typical spoilage-causing and pathogenic bacteria found in meat and meat products. The highest total phenolic content (TPC) was detected in extract, followed by and extracts. The antioxidant capacity of the extracts was measured by DPPH and ABTS assays. The lowest IC values were found for extract, followed by and extracts. LC-MS and HPLC analysis revealed that and extracts contained hydroxycinnamic acid derivatives and flavonoids (mainly flavonols). Hydroxycinnamic acid derivatives were detected in the extract, as well as flavonols, ellagitannins, and iridoids. The antibacterial activity of the plant extracts was tested against Gram-negative bacteria (, , , , , ) and Gram-positive bacteria (, , , , ) using the microculture method. The extracts acted as bacteriostatic agents, decreasing the growth rate (µ) and extending the lag phase (t). showed most potent antibacterial activity, as confirmed by principal component analysis (PCA).

摘要

本研究的目的是调查[植物名称1]、[植物名称2]和[植物名称3]叶片提取物的成分,以及它们对肉类和肉类产品中典型的致腐和致病细菌的抗菌活性。在[植物名称1]提取物中检测到最高的总酚含量(TPC),其次是[植物名称2]和[植物名称3]提取物。通过DPPH和ABTS测定法测量提取物的抗氧化能力。在[植物名称1]提取物中发现最低的IC值,其次是[植物名称2]和[植物名称3]提取物。LC-MS和HPLC分析表明,[植物名称2]和[植物名称3]提取物含有羟基肉桂酸衍生物和黄酮类化合物(主要是黄酮醇)。在[植物名称1]提取物中检测到羟基肉桂酸衍生物,以及黄酮醇、鞣花单宁和环烯醚萜。使用微量培养法测试植物提取物对革兰氏阴性菌([细菌名称1]、[细菌名称2]、[细菌名称3]、[细菌名称4]、[细菌名称5]、[细菌名称6])和革兰氏阳性菌([细菌名称7]、[细菌名称8]、[细菌名称9]、[细菌名称10])的抗菌活性。提取物起到抑菌剂的作用,降低生长速率(µ)并延长延迟期(t)。主成分分析(PCA)证实,[植物名称1]表现出最强的抗菌活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5afc/7248868/7360282551bb/molecules-25-02011-g001.jpg

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