Beauvillain J C, Mazzuca M, Dubois M P
Cell Tissue Res. 1977 Nov 7;184(3):343-58. doi: 10.1007/BF00219895.
Different immunohistochemical techniques were used to identify the prolactin and growth hormone producing cells (STH cells) in the pituitary of normal guinea pigs at the ultrastructural level. Prolactin cells revealed two main aspects: 1. Cells with granules from 2500 to 3500 A in diameter some of which are irregularly shaped. These granulations are scattered throughout the whole cytoplasm. The rough endoplasmic reticulum is well developed and organized in parallel lamellae. 2. In cells of the second type the granules are less numerous and smaller in diameter (1800 to 2500 A) and the rough endoplasmic reticulum is less well developed. The cytoplasm of the STH cells contains many more round granules (generally grouped) which also range from 2500 to 3500 A in diameter. The prolactin molecules and the STH molecules are essentially confined to the granules but with the immunocytochemical technique before embedding a slightly diffuse reaction appeared in the entire cytoplasm. These results are discussed.
采用不同的免疫组织化学技术，在超微结构水平上鉴定正常豚鼠垂体中分泌催乳素和生长激素的细胞（促生长激素细胞）。催乳素细胞呈现出两个主要特征：1. 细胞内含有直径为2500至3500埃的颗粒，其中一些颗粒形状不规则。这些颗粒散布于整个细胞质中。粗面内质网发达，呈平行板层状排列。2. 第二类细胞中的颗粒数量较少，直径较小（1800至2500埃），粗面内质网发育较差。促生长激素细胞的细胞质中含有更多圆形颗粒（通常成组存在），其直径也在2500至3500埃之间。催乳素分子和促生长激素分子主要局限于颗粒中，但在包埋前的免疫细胞化学技术检测中，整个细胞质中出现了轻微的弥散反应。对这些结果进行了讨论。
