Beauvillain J C, Mazzuca M, Dubois M P
Cell Tissue Res. 1976 Oct 29;174(2):233-44. doi: 10.1007/BF00222161.
Three different immunocytoenzymatic techniques were used to identify and characterize the thyrotropic cells in the pituitary of normal guinea pigs at the ultrastructural level (superimposition technique, immunocytochemical technique using P.A.P. and indirect immunohistoenzymatic method before embedding). These cells are characterized by a dark cytoplasm with granules ranging from 1500 to 2000 A in diameter. The appearance of these granules is very variable: some display a marked electron density and are homogeneous but some have a less marked electron density with a more electron dense peripherally situated region. The TSH molecules are essentially confined to the granules but when the immunocytochemical reactions are carried out before embedding, positive staining is also seen in the cytoplasm and the outer surface of most of the rough endoplasmic reticulum membranes. These results are discussed.
采用三种不同的免疫细胞酶技术，在超微结构水平上（叠加技术、使用过氧化物酶抗过氧化物酶法的免疫细胞化学技术以及包埋前间接免疫组织酶法）对正常豚鼠垂体中的促甲状腺细胞进行鉴定和特征描述。这些细胞的特征是细胞质深色，含有直径为1500至2000埃的颗粒。这些颗粒的外观变化很大：有些显示出明显的电子密度且均匀一致，但有些电子密度不太明显，周边区域电子密度更高。促甲状腺激素分子主要局限于颗粒中，但在包埋前进行免疫细胞化学反应时，在细胞质以及大多数粗面内质网膜的外表面也可见阳性染色。对这些结果进行了讨论。
