Li J Y, Dubois M P, Dubois P M
Cell Tissue Res. 1977 Jul 19;181(4):545-52. doi: 10.1007/BF00221775.
An immunocytochemical staining of ultrathin sections using the peroxidase-antiperoxidase complex was employed to localize the somatotrophs in the anterior pituitary of the human fetus. This technique allowed a specific identification of the somatotrophs and a detailed description of their ultrastructure which was well preserved by the fixation employed. They appeared as cells with a dense cytoplasmic matrix, lamellar rough endoplasmic reticulum, and prominent Golgi apparatus. Their secretory granules had a mean diameter of 300 nm.
采用过氧化物酶 - 抗过氧化物酶复合物对超薄切片进行免疫细胞化学染色，以定位人类胎儿垂体前叶中的生长激素细胞。该技术能够特异性识别生长激素细胞，并详细描述其超微结构，所采用的固定方法能很好地保存这些结构。它们表现为具有致密细胞质基质、板层状粗面内质网和显著高尔基体的细胞。其分泌颗粒的平均直径为300纳米。
