Marin-Lopez Alejandro, Wang Yuchen, Jiang Junjun, Ledizet Michel, Fikrig Erol
Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA.
Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA; State Key Laboratory of Virology, College of Life Science, Wuhan University, Wuhan, Hubei 430072, China.
Vaccine. 2021 Mar 19;39(12):1675-1679. doi: 10.1016/j.vaccine.2021.01.072. Epub 2021 Feb 20.
Zika virus(ZIKV) is primarily spread by Aedes. aegyptimosquitoes. Infection with ZIKV can result in diverse clinical symptoms in humans, ranging from mild to severe. Previously, we demonstrated that passive immunization against A. aegypti AgBR1 or NeSt1 antiserum, two mosquito saliva proteins that are transmitted with the virus, conferred partial protection against ZIKV in mice. Each individual antiserum altered the early host response in the skin and reduced viremia. Here, we show that passive immunization with a combination of AgBR1- and NeSt1-specific antibodies enhanced survival and reduced the viral burden in blood, thereby protecting mice from mosquito-borne ZIKV infection. This finding suggests that targeting a combination of mosquito saliva proteins, with AgBR1 and NeSt1 as model antigens, may be used as a vaccine strategy to help prevent mosquito-borne ZIKV infection.
寨卡病毒（ZIKV）主要通过埃及伊蚊传播。人类感染ZIKV可导致从轻度到重度的多种临床症状。此前，我们证明，针对埃及伊蚊AgBR1或NeSt1抗血清（两种随病毒传播的蚊虫唾液蛋白）进行被动免疫，可在小鼠中对ZIKV提供部分保护。每种单独的抗血清都会改变皮肤中的早期宿主反应并降低病毒血症。在此，我们表明，用AgBR1特异性抗体和NeSt1特异性抗体的组合进行被动免疫可提高存活率并降低血液中的病毒载量，从而保护小鼠免受蚊媒ZIKV感染。这一发现表明，以AgBR1和NeSt1作为模型抗原，针对多种蚊虫唾液蛋白的组合可能用作一种疫苗策略，以帮助预防蚊媒ZIKV感染。
