Caldwell C W, Barrett J T
Clin Chim Acta. 1977 Dec 15;81(3):305-9. doi: 10.1016/0009-8981(77)90065-1.
A solid-phase enzyme immunoassay for the qualitative detection of hepatitis B surface antigenemia is presented and the results compared to the counter-immunoelectrophoresis and radioimmunoassay methods for detection of the same antigen. The enzyme-antibody conjugate was prepared from horseradish peroxidase coupled with antibodies to three sub-types of hepatitis B virus. Polystyrene plastic tubes were used as the solid-phase support. The results of the enzyme immunoassay compare favorably with radioimmunoassay and exceed counterimmunoelectrophoresis in sensitivity.
