Zhou C, Zheng X, Huang X, Su J, Li M, Chen Z, Li M, Chi H
First Clinical Medical College, Guangdong Medical University, Zhanjiang 524023, China.
School Of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2021 Feb 25;41(2):256-263. doi: 10.12122/j.issn.1673-4254.2021.02.14.
To explore the effect of decoction (HQD) on group 3 innate lymphoid cells (ILC3s) and helper T cells (Th) for treatment of ulcerative colitis (UC).
Male Balb/c mice were randomly divided into control group, DSS group, mesalazine group (ME, 400 mg/kg), and 2.275 g/kg, 4.55 g/kg and 9.1 g/kg HQD groups. All the mice were given free access to normal chow. Except for those in the normal control group, all the mice were given 3% DSS solution for 7 days to establish models of UC. The mice in ME group and 3 HQD groups were given mesalazine or HQD via oral gavage at the specified doses once a day. Flow cytometry was performed to analyze the ILC3s, MHC Ⅱ, Th1 and Treg in the lamina propria lymphocytes in the colon. Milliplex was performed to determine cytokine levels of in the colon tissues.
Compared with those in DSS group, the mice in the 3 HQD groups all showed obviously lessened symptoms of UC with significantl decreased DAI score ( < 0.001) and macroscopic score ( < 0.001). The results of flow cytometry showed that HQD treatment significantly increased the percentage of ILC3s ( < 0.05) and expression of MHCⅡ ( < 0.05), obviously reduced the proportion of Th1 ( < 0.05) but increased Treg cells ( < 0.05) in the colon tissues. Milliplex showed that HQD treatment significantly reduced the expressions of Th-related pro-inflammatory cytokines including IL-2 ( < 0.05), IL-17A ( < 0.05), IL-23 ( < 0.05), TNF-α ( < 0.05), and IFN-γ ( < 0.05).
HQD alleviates DSS- induced UC in mice by increasing ILC3s and MHC Ⅱ expression to suppress the function of Th17 and Th1 cells and promote Treg and Th2 cells.
探讨黄芪汤（HQD）对3型天然淋巴细胞（ILC3s）和辅助性T细胞（Th）的影响，以用于治疗溃疡性结肠炎（UC）。
将雄性Balb/c小鼠随机分为对照组、葡聚糖硫酸钠（DSS）组、美沙拉嗪组（ME，400mg/kg）以及2.275g/kg、4.55g/kg和9.1g/kg的HQD组。所有小鼠均可自由摄取普通食物。除正常对照组小鼠外，其余小鼠均给予3% DSS溶液，持续7天以建立UC模型。ME组和3个HQD组的小鼠每天经口灌胃给予指定剂量的美沙拉嗪或HQD。采用流式细胞术分析结肠固有层淋巴细胞中的ILC3s、主要组织相容性复合体Ⅱ类分子（MHCⅡ）、Th1和调节性T细胞（Treg）。采用多重免疫分析方法测定结肠组织中的细胞因子水平。
与DSS组相比，3个HQD组小鼠的UC症状均明显减轻，疾病活动指数（DAI）评分（<0.001）和宏观评分（<0.001）显著降低。流式细胞术结果显示，HQD治疗显著提高了结肠组织中ILC3s的百分比（<0.05）和MHCⅡ的表达（<0.05），明显降低了Th1的比例（<0.05），但增加了Treg细胞（<0.05）。多重免疫分析显示，HQD治疗显著降低了包括白细胞介素-2（IL-2，<0.05）、白细胞介素-17A（IL-17A，<0.05）、白细胞介素-23（IL-23，<0.05）、肿瘤坏死因子-α（TNF-α，<0.05）和干扰素-γ（IFN-γ，<0.05）在内的Th相关促炎细胞因子的表达。
HQD通过增加ILC3s和MHCⅡ的表达来抑制Th17和Th1细胞的功能，并促进Treg和Th辅助细胞2（Th2）细胞，从而减轻DSS诱导的小鼠UC。
