CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Multi-disciplinary Research Division, Institute of High Energy Physics and University of Chinese Academy of Sciences (UCAS), Chinese Academy of Sciences (CAS), Beijing 100049, P. R. China.
Chem Commun (Camb). 2021 Mar 25;57(24):3038-3041. doi: 10.1039/d0cc08316j. Epub 2021 Feb 24.
The DNAzyme activity of G-quadruplex/hemin in mitochondria has not been characterized. Herein, we report an unexpected difference in the DNAzyme activity between in vitro assays and in mitochondria. Molecular dynamic simulations illustrate how the interaction of the G-quadruplex with hemin may modulate the DNAzyme activity. These results might facilitate a better understanding of the catalytic mechanism of the DNAzyme and help the rational design of stable and active DNAzymes suitable for intracellular catalysis.
线粒体中 G-四链体/血红素的 DNA 酶活性尚未得到表征。在此，我们报告了体外测定和线粒体中 DNA 酶活性之间出人意料的差异。分子动力学模拟说明了 G-四链体与血红素的相互作用如何调节 DNA 酶的活性。这些结果可能有助于更好地理解 DNA 酶的催化机制，并有助于设计适合细胞内催化的稳定且具有活性的 DNA 酶。
