血红素与四链体DNA的相互作用。
Interaction of hemin with quadruplex DNA.
作者信息
Ghahremani Nasab Maryam, Hassani Leila, Mohammadi Nejad Sara, Norouzi Davood
机构信息
Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, 45195-1159, Iran.
Laboratory of Cell Biology, National Cancer Institute NIH, Bethesda, MD, 20892, USA.
出版信息
J Biol Phys. 2017 Mar;43(1):5-14. doi: 10.1007/s10867-016-9430-7. Epub 2016 Oct 17.
A DNA enzyme with peroxidase activity is a G-quadruplex-based DNAzyme formed by hemin and G-quadruplex DNA. Activity of peroxide DNAzymes can be influenced by the structure of quadruplex DNA. In this investigation, the interaction of hemin with T30695 G-quadruplex DNA is evaluated. Molecular dynamic simulation indicates that the binding mode of hemin to G-quadruplex DNA is end-stacking, which is consistent with absorption spectroscopy. Based on fluorescence spectroscopy, hemin ejects thiazole orange from bases of four-strand DNA. Circular dichroism spectra showed that no alteration occurs in this type of DNA structure. Graphical Abstract Peroxidase DNAzyme is formed by hemin and G-quadruplex DNA.
一种具有过氧化物酶活性的DNA酶是由血红素和G-四链体DNA形成的基于G-四链体的DNA酶。过氧化物DNA酶的活性会受到四链体DNA结构的影响。在本研究中，评估了血红素与T30695 G-四链体DNA的相互作用。分子动力学模拟表明，血红素与G-四链体DNA的结合模式是末端堆积，这与吸收光谱一致。基于荧光光谱，血红素从四链DNA的碱基中逐出噻唑橙。圆二色光谱表明，这种类型的DNA结构没有发生改变。图形摘要：过氧化物酶DNA酶由血红素和G-四链体DNA形成。
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