Institute of Bioengineering, Guangdong Academy of Sciences, Guangzhou, Guangdong, China.
Key Laboratory of Natural Pesticides and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, Guangdong, China.
PLoS One. 2021 Feb 24;16(2):e0247127. doi: 10.1371/journal.pone.0247127. eCollection 2021.
The cultivation and production of passion fruit (Passiflora edulis) are severely affected by viral disease. Yet there have been few studies of the molecular response of passion fruit to virus attack. In the present study, RNA-based transcriptional profiling (RNA-seq) was used to identify the gene expression profiles in yellow passion fruit (Passiflora edulis f. flavicarpa) leaves following inoculation with cucumber mosaic virus (CMV). Six RNA-seq libraries were constructed comprising a total of 42.23 Gb clean data. 1,545 differentially expressed genes (DEGs) were obtained (701 upregulated and 884 downregulated). Gene annotation analyses revealed that genes associated with plant hormone signal transduction, transcription factors, protein ubiquitination, detoxification, phenylpropanoid biosynthesis, photosynthesis and chlorophyll metabolism were significantly affected by CMV infection. The represented genes activated by CMV infection corresponded to transcription factors WRKY family, NAC family, protein ubiquitination and peroxidase. Several DEGs encoding protein TIFY, pathogenesis-related proteins, and RNA-dependent RNA polymerases also were upregualted by CMV infection. Overall, the information obtained in this study enriched the resources available for research into the molecular-genetic mechanisms of the passion fruit/CMV interaction, and might provide a theoretical basis for the prevention and management of passion fruit viral disease in the field.
百香果的种植和生产受到病毒病的严重影响。然而，对百香果受病毒攻击的分子反应的研究很少。在本研究中，基于 RNA 的转录组学（RNA-seq）用于鉴定接种黄瓜花叶病毒（CMV）后黄百香果（Passiflora edulis f. flavicarpa）叶片的基因表达谱。构建了 6 个 RNA-seq 文库，共包含 42.23 Gb 清洁数据。获得了 1545 个差异表达基因（DEGs）（701 个上调和 884 个下调）。基因注释分析表明，与植物激素信号转导、转录因子、蛋白质泛素化、解毒、苯丙烷生物合成、光合作用和叶绿素代谢相关的基因受 CMV 感染的显著影响。CMV 感染激活的代表性基因对应于 WRKY 家族、NAC 家族、蛋白质泛素化和过氧化物酶的转录因子。一些编码蛋白 TIFY、病程相关蛋白和 RNA 依赖性 RNA 聚合酶的 DEGs 也被 CMV 感染上调。总的来说，本研究获得的信息丰富了百香果/CMV 相互作用的分子遗传机制研究资源，并可为田间百香果病毒病的防治提供理论依据。
