Alves Murilo S, Dadalto Silvana P, Gonçalves Amanda B, de Souza Gilza B, Barros Vanessa A, Fietto Luciano G
Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Viçosa, Minas Gerais 36570000, Brazil.
Proteomes. 2014 Mar 4;2(1):85-106. doi: 10.3390/proteomes2010085.
Responses to biotic stress in plants lead to dramatic reprogramming of gene expression, favoring stress responses at the expense of normal cellular functions. Transcription factors are master regulators of gene expression at the transcriptional level, and controlling the activity of these factors alters the transcriptome of the plant, leading to metabolic and phenotypic changes in response to stress. The functional analysis of interactions between transcription factors and other proteins is very important for elucidating the role of these transcriptional regulators in different signaling cascades. In this review, we present an overview of protein-protein interactions for the six major families of transcription factors involved in plant defense: basic leucine zipper containing domain proteins (bZIP), amino-acid sequence WRKYGQK (WRKY), myelocytomatosis related proteins (MYC), myeloblastosis related proteins (MYB), APETALA2/ ETHYLENE-RESPONSIVE ELEMENT BINDING FACTORS (AP2/EREBP) and no apical meristem (NAM), Arabidopsis transcription activation factor (ATAF), and cup-shaped cotyledon (CUC) (NAC). We describe the interaction partners of these transcription factors as molecular responses during pathogen attack and the key components of signal transduction pathways that take place during plant defense responses. These interactions determine the activation or repression of response pathways and are crucial to understanding the regulatory networks that modulate plant defense responses.
植物对生物胁迫的反应会导致基因表达的剧烈重编程，以牺牲正常细胞功能为代价来促进胁迫反应。转录因子是转录水平上基因表达的主要调节因子，控制这些因子的活性会改变植物的转录组，从而导致植物在应对胁迫时发生代谢和表型变化。对转录因子与其他蛋白质之间相互作用的功能分析，对于阐明这些转录调节因子在不同信号级联反应中的作用非常重要。在本综述中，我们概述了参与植物防御的六大类转录因子的蛋白质-蛋白质相互作用：含碱性亮氨酸拉链结构域蛋白（bZIP）、氨基酸序列为WRKYGQK的蛋白（WRKY）、与髓细胞瘤相关的蛋白（MYC）、与成髓细胞血症相关的蛋白（MYB）、APETALA2/乙烯响应元件结合因子（AP2/EREBP）以及无顶端分生组织（NAM）、拟南芥转录激活因子（ATAF）和杯状子叶（CUC）（NAC）。我们将这些转录因子的相互作用伙伴描述为病原体攻击期间的分子反应以及植物防御反应期间发生的信号转导途径的关键组成部分。这些相互作用决定了反应途径的激活或抑制，对于理解调节植物防御反应的调控网络至关重要。
