Department of Oral and Maxillofacial Implantology, Tokyo Dental College, 2-9-18 Kanda misaki-cho, Chiyoda-ku, Tokyo, 101-0061, Japan.
Oral Health Science Center, Tokyo Dental College, 2-9-18 Kanda misaki-cho, Chiyoda-ku, Tokyo, Japan.
Sci Rep. 2021 Feb 24;11(1):4498. doi: 10.1038/s41598-021-84096-x.
Streptococci are associated with dental plaque formation as the early-colonizing bacteria that adhere to titanium (CpTi) and zirconia (TZP) implant abutment surfaces. Effective prevention of peri-implantitis may be possible by removing streptococci as target. This study aimed to evaluate the effects of 2% NaF on the prevention of streptococcal adhesion to CpTi and TZP. After immersion in 2% NaF for 90 min, surface characterization of mirror-polished CpTi and TZP disks were assesed using XPS, EPMA, and SEM. S. sanguinis, S. gordonii, and S. oralis were used as the streptococcal bacterial strains. After 24 h culture, bacterial adhesion was evaluated using an ATP-bioluminescent assay and SEM. In XPS, EPMA, and SEM analyses, fluoride was detected on the CpTi and TZP surfaces after 2% NaF immersion with no signs of localization, and no corrosion on the CpTi disks. Based on the adhesion assay, the adherences of S. sanguinis, S. gordonii, and S. oralis were significantly lower with NaF than without NaF in CpTi (p = 0.005, 0.001, and 0.001, respectively) and TZP (p = 0.003, 0.002, and 0.001). This was also confirmed by SEM. In conclusion, 2% NaF reduced the adhesion of streptococci to the CpTi and TZP surfaces.
链球菌与牙菌斑的形成有关，是早期定植于钛（CpTi）和氧化锆（TZP）种植体基台表面的细菌。通过去除链球菌作为靶点，可能可以有效地预防种植体周围炎。本研究旨在评估 2%氟化钠对预防链球菌黏附于 CpTi 和 TZP 的效果。将经过镜面抛光的 CpTi 和 TZP 圆盘浸入 2%氟化钠中 90 分钟后，使用 XPS、EPMA 和 SEM 评估其表面特性。将 S. sanguinis、S. gordonii 和 S. oralis 用作链球菌细菌株。在 24 小时培养后，使用 ATP 生物发光法和 SEM 评估细菌黏附。在 XPS、EPMA 和 SEM 分析中，在 2%氟化钠浸泡后，在 CpTi 和 TZP 表面检测到氟化物，没有定位迹象，且 CpTi 圆盘没有腐蚀。根据黏附试验，与无氟化钠相比，2%氟化钠可显著降低 S. sanguinis、S. gordonii 和 S. oralis 在 CpTi（p=0.005、0.001 和 0.001）和 TZP（p=0.003、0.002 和 0.001）表面的黏附。这也通过 SEM 得到了证实。总之，2%氟化钠减少了链球菌对 CpTi 和 TZP 表面的黏附。
