Department of Cardiology, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders (Chongqing), China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, People's Republic of China.
Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University, Chongqing, 400014, People's Republic of China.
Int J Nanomedicine. 2021 Feb 17;16:1331-1344. doi: 10.2147/IJN.S293736. eCollection 2021.
Myocardial infarction (MI) is a serious threat to public health. The early identification of MI is important to promote appropriate treatment strategies for patients. Recently, strategies targeting extracellular matrix (ECM) components have gained attention. Fibrin is an ECM protein involved after MI. In this work, we constructed fibrin-targeted nanoparticles (NPs) by co-assembling a fibrin-targeted peptide (CREKA) and indocyanine green (ICG) and used them to enhance photoacoustic (PA) imaging for noninvasive detection of the infarct region to help diagnose MI.
ICG NPs modified with CREKA were prepared (CREKA-ICG-LIP NPs). Then, the fundamental characteristics, stability, safety, and targeting ability of the NPs were detected. Finally, in an ischemia-reperfusion (IR) injury model, the performance of the NPs in detecting the infarct region in the model on PA imaging was evaluated.
CREKA-ICG-LIP NPs were successfully constructed and showed excellent basic characteristics, a high safety level, and an excellent targeting ability. After intravenous injection, the CREKA-ICG-LIP NPs accumulated in the injured region in the IR model. Then, the PA signal in the infarct region could be detected by the ultrasound transducer of the Vevo LAZR Photoacoustic Imaging System.
This work provides new insights for non-invasive, real-time imaging techniques to detect the region of myocardial injury and help diagnose MI based on a PA imaging system with high sensitivity in optical imaging and deep penetration in ultrasound imaging.
心肌梗死（MI）是对公众健康的严重威胁。早期识别 MI 对于为患者提供适当的治疗策略很重要。最近，针对细胞外基质（ECM）成分的策略受到了关注。纤维蛋白是 MI 后涉及的 ECM 蛋白。在这项工作中，我们通过共组装纤维蛋白靶向肽（CREKA）和吲哚菁绿（ICG）构建了纤维蛋白靶向纳米颗粒（NPs），并将其用于增强光声（PA）成像，以无创检测梗死区域，帮助诊断 MI。
制备了修饰有 CREKA 的 ICG NPs（CREKA-ICG-LIP NPs）。然后，检测了 NPs 的基本特性、稳定性、安全性和靶向能力。最后，在缺血再灌注（IR）损伤模型中，评估了 NPs 在 PA 成像模型中检测模型中梗死区域的性能。
成功构建了 CREKA-ICG-LIP NPs，表现出优异的基本特性、高安全性和优异的靶向能力。静脉注射后，CREKA-ICG-LIP NPs 在 IR 模型中的损伤区域聚集。然后，通过 Vevo LAZR 光声成像系统的超声换能器可以检测到梗死区域的 PA 信号。
这项工作为基于具有光学成像高灵敏度和超声成像深穿透能力的 PA 成像系统的无创、实时成像技术提供了新的见解，以检测心肌损伤区域并帮助诊断 MI。
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