Abebe Dehnnet, Periasamy Gomathi, Karim Aman, Bitew Helen
Debre Markos University, Debre Markos, Ethiopia.
Mekelle University, Mekelle, Ethiopia.
Evid Based Complement Alternat Med. 2021 Feb 10;2021:1838903. doi: 10.1155/2021/1838903. eCollection 2021.
(Steud. ex A. Rich) Descoings ex wild & Drummond (Vitaceae) has been used in traditional medicine for the management of various immunological and hematological disorders in different areas of Ethiopia and the rest of the world. In Ethiopia, the plant is used for the management of enlarged spleen, rabies virus, helminthic infection, snake bite, and various types of tumors.
To evaluate the effect of hydroethanolic root extract and solvent fractions of on cell-mediated immunity (delayed-type hypersensitivity), organ index (spleen and liver), and blood cell count in Swiss albino mice.
Acute oral toxicity test was conducted using nulliparous and nonpregnant Swiss albino mice following OECD 425 limit test method. Delayed-type hypersensitivity model was used to evaluate the effect on cell-mediated immunity. The experimental animals were divided into twelve groups which were sensitized and challenged with sheep red blood cells on day 0 and day 7, respectively. Levamisole 50 mg/kg was used as stimulant control, whereas cyclophosphamide 30 mg/kg was used as suppressant control. Hydroethanolic root extract (100 mg/kg, 200 mg/kg, and 400 mg/kg), aqueous fraction (100 mg/kg, 200 mg/kg, and 400 mg/kg), and butanol fraction (100 mg/kg, 200 mg/kg, and 400 mg/kg) were administered for seven days. The paw volume was measured using a digital plethysmometer before challenge and 24 hours after challenge. Blood was collected, and organs (spleen and liver) were isolated from each challenged mouse to determine blood cell count and organ index, respectively.
No mortality and noticeable behavioral changes were observed among all mice receiving hydroethanolic root extract and solvent fractions at a dose of 2000 mg/kg. Hydroethanolic root extract and solvent fractions of showed enhancement of delayed-type hypersensitivity, organ index, and blood cell count. Hydroethanolic root extract at a dose of 400 mg/kg showed the highest and statistically significant stimulation of delayed-type hypersensitivity (0.123 ± 0.010) and blood cell count compared to the vehicle.
Hydroethanolic root extract and solvent fractions of showed a stimulatory effect on cell-mediated immunity and hematopoiesis.
（源自A. Rich的Steud.）Descoings ex wild & Drummond（葡萄科）在埃塞俄比亚不同地区及世界其他地方的传统医学中被用于治疗各种免疫和血液系统疾病。在埃塞俄比亚，该植物用于治疗脾脏肿大、狂犬病病毒、蠕虫感染、蛇咬伤以及各种类型的肿瘤。
评估Descoings ex wild & Drummond的水乙醇根提取物及其溶剂部位对瑞士白化小鼠细胞介导免疫（迟发型超敏反应）、器官指数（脾脏和肝脏）及血细胞计数的影响。
按照经合组织425限量试验方法，对未生育且未怀孕的瑞士白化小鼠进行急性经口毒性试验。采用迟发型超敏反应模型评估对细胞介导免疫的影响。将实验动物分为12组，分别在第0天和第7天用绵羊红细胞进行致敏和激发。左旋咪唑50mg/kg用作刺激对照，环磷酰胺30mg/kg用作抑制对照。给予水乙醇根提取物（100mg/kg、200mg/kg和400mg/kg）、水部位（100mg/kg、200mg/kg和400mg/kg）和正丁醇部位（100mg/kg、200mg/kg和400mg/kg），持续7天。在激发前和激发后24小时，使用数字体积描记器测量爪体积。采集血液，并从每只激发后的小鼠中分离出器官（脾脏和肝脏），分别测定血细胞计数和器官指数。
在所有接受2000mg/kg剂量水乙醇根提取物及其溶剂部位的小鼠中，未观察到死亡和明显的行为变化。Descoings ex wild & Drummond的水乙醇根提取物及其溶剂部位显示出迟发型超敏反应、器官指数和血细胞计数的增强。与赋形剂相比，400mg/kg剂量的水乙醇根提取物对迟发型超敏反应（0.123±0.010）和血细胞计数具有最高且具有统计学意义的刺激作用。
Descoings ex wild & Drummond的水乙醇根提取物及其溶剂部位对细胞介导免疫和造血具有刺激作用。
