Zhou Caibi, Zhou Xiaolu, Wen Zhirui, Yang Zaibo, Mu Ren, Song Yuyan, Mei Xin, Rothenberg Dylan O'Neill
College of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, China.
College of Horticulture and Landscape Architecture, Hunan Agricultural University, Changsha 410000, China.
Evid Based Complement Alternat Med. 2021 Feb 9;2021:8832554. doi: 10.1155/2021/8832554. eCollection 2021.
Intake of a high-fat diet (HFD) is closely related to disorders of the intestinal microbiota, which plays a key role in the pathogenesis of obesity. Duyun compound green tea, an ancient Chinese drink, is widely consumed to reduce weight, although the mechanism is not clear. In this study, 50 mice were randomly divided into 5 groups: normal control group (CK), HFD model control group (NK), positive control group with medicine (YK), low-dose compound tea group (DL), and high-dose compound tea group (DH). After 4 weeks of intervention, the feces of mice were taken under sterile conditions and evaluated using Illumina high-throughput sequencing technology. The results showed that the diversity of intestinal microbiota was the highest in the CK group, the lowest in the NK group, and relatively increased in the compound tea treatment group. Second, there were differences in intestinal microbiota in each group, among which the beneficial bacteria in the intestinal tract of the CK group were higher than those in the other groups, while the beneficial bacteria in each compound tea treatment group were more abundant than those in the NK group, in which harmful bacteria in the intestinal tract were found to be the highest. These results suggest that compounds in tea may be able to attenuate imbalances of intestinal microbiota induced by poor diet, acting as a therapeutic agent in obesity or other diseases associated with gut dysbiosis.
高脂饮食（HFD）的摄入与肠道微生物群紊乱密切相关，肠道微生物群在肥胖发病机制中起关键作用。都匀复方绿茶是一种中国古代饮品，虽作用机制尚不清楚，但被广泛用于减肥。本研究将50只小鼠随机分为5组：正常对照组（CK）、高脂饮食模型对照组（NK）、药物阳性对照组（YK）、低剂量复方茶组（DL）和高剂量复方茶组（DH）。干预4周后，在无菌条件下采集小鼠粪便，并采用Illumina高通量测序技术进行评估。结果显示，肠道微生物群多样性在CK组最高，在NK组最低，在复方茶治疗组相对增加。其次，各组肠道微生物群存在差异，其中CK组肠道有益菌高于其他组，而各复方茶治疗组有益菌比NK组更丰富，NK组肠道有害菌最高。这些结果表明，茶中的化合物可能能够减轻不良饮食引起的肠道微生物群失衡，在肥胖或其他与肠道菌群失调相关的疾病中起到治疗作用。
