Chen Lin, Jiang Qihong, Lu Hongling, Jiang Chenkai, Hu Wenjun, Liu Hanxiao, Xiang Xingwei, Tan Chin Ping, Zhou Tianhuan, Shen Guoxin
Institute of Sericultural and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Zhejiang Feida Environmental Science & Technology Co., Ltd., Shaoxing 311800, China.
Foods. 2024 Jul 26;13(15):2352. doi: 10.3390/foods13152352.
Obesity has become one of the most serious chronic diseases threatening human health. Its onset and progression are closely related to the intestinal microbiota, as disruption of the intestinal flora promotes the production of endotoxins and induces an inflammatory response. This study aimed to investigate the variations in the physicochemical properties of various refined tea seed oils and their impact on intestinal microbiota disorders induced by a high-fat diet (HFD) through dietary intervention. In the present study, C57BL/6J mice on a HFD were randomly divided into three groups: HFD, T-TSO, and N-TSO. T-TSO and N-TSO mice were given traditionally refined and optimized tea seed oil for 12 weeks. The data revealed that tea seed oil obtained through degumming at 70 °C, deacidification at 50 °C, decolorization at 90 °C, and deodorization at 180 °C (at 0.06 MPa for 1 h) effectively removed impurities while minimizing the loss of active ingredients. Additionally, the optimized tea seed oil mitigated fat accumulation and inflammatory responses resulting from HFD, and reduced liver tissue damage in comparison to traditional refining methods. More importantly, N-TSO can serve as a dietary supplement to enhance the diversity and abundance of intestinal microbiota, increasing the presence of beneficial bacteria (, , and ) while reducing pathogenic bacteria ( and ). Therefore, in HFD-induced obese C57BL/6J mice, N-TSO can better ameliorate obesity compared with a T-TSO diet, which is promising in alleviating HFD-induced intestinal microbiota disorders.
肥胖已成为威胁人类健康的最严重慢性疾病之一。其发病和进展与肠道微生物群密切相关，因为肠道菌群的破坏会促进内毒素的产生并引发炎症反应。本研究旨在通过饮食干预，研究各种精制茶籽油的理化性质变化及其对高脂饮食（HFD）诱导的肠道微生物群紊乱的影响。在本研究中，将高脂饮食的C57BL/6J小鼠随机分为三组：HFD组、T-TSO组和N-TSO组。给T-TSO组和N-TSO组小鼠喂食传统精制和优化的茶籽油，持续12周。数据显示，通过在70℃脱胶、50℃脱酸、90℃脱色和180℃（0.06MPa，1小时）脱臭获得的茶籽油能有效去除杂质，同时将活性成分的损失降至最低。此外，与传统精炼方法相比，优化后的茶籽油减轻了高脂饮食导致的脂肪堆积和炎症反应，并减少了肝组织损伤。更重要的是，N-TSO可作为一种膳食补充剂，增强肠道微生物群的多样性和丰度，增加有益菌（、和）的数量，同时减少病原菌（和）。因此，在高脂饮食诱导的肥胖C57BL/6J小鼠中，与T-TSO饮食相比，N-TSO能更好地改善肥胖，这在缓解高脂饮食诱导的肠道微生物群紊乱方面具有前景。
