Zander N F, Meyer H E, Hoffmann-Posorske E, Crabb J W, Heilmeyer L M, Kilimann M W
Institut für Physiologische Chemie, Ruhr-Universität Bochum, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1988 May;85(9):2929-33. doi: 10.1073/pnas.85.9.2929.
We have isolated and sequenced a cDNA encoding the alpha subunit of phosphorylase kinase from rabbit fast-twitch skeletal muscle. The cDNA molecule consists of 388 nucleotides of 5'-nontranslated sequence, the complete coding sequence of 3711 nucleotides, and 342 nucleotides of 3'-nontranslated sequence followed by a poly(dA) tract. It encodes a polypeptide of 1237 amino acids and a deduced molecular mass of 138,422 Da. Nearly half of the deduced amino acid sequence is confirmed by peptide sequencing. Seven positions of endogenously phosphorylated serine residues and autophosphorylation sites, identified by peptide sequencing, could be assigned. They cluster in a segment of only 60 amino acids. RNA blot hybridization analysis demonstrates a predominant RNA species of approximately equal to 4500 nucleotides and a less abundant RNA of 8700 nucleotides.
我们已从兔快肌骨骼肌中分离并测序了编码磷酸化酶激酶α亚基的cDNA。该cDNA分子由388个核苷酸的5'-非翻译序列、3711个核苷酸的完整编码序列以及342个核苷酸的3'-非翻译序列组成，后面跟着一个聚腺苷酸尾。它编码一个由1237个氨基酸组成的多肽，推导分子量为138,422道尔顿。推导的氨基酸序列中近一半通过肽测序得到证实。通过肽测序鉴定出的7个内源性磷酸化丝氨酸残基和自磷酸化位点可以确定。它们聚集在仅60个氨基酸的一段序列中。RNA印迹杂交分析显示一种主要的约4500个核苷酸的RNA种类和一种较少的8700个核苷酸的RNA。
