• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

编码磷酸化酶激酶完整催化亚基的cDNA克隆的分离与序列分析

Isolation and sequence analysis of a cDNA clone encoding the entire catalytic subunit of phosphorylase kinase.

作者信息

da Cruz e Silva E F, Cohen P T

出版信息

FEBS Lett. 1987 Aug 10;220(1):36-42. doi: 10.1016/0014-5793(87)80871-2.

DOI:10.1016/0014-5793(87)80871-2
PMID:3609320
Abstract

Synthetic oligonucleotides have been used to isolate a 1.85 kb clone containing the full length coding sequence for the catalytic subunit of rabbit skeletal muscle phosphorylase kinase from a cDNA library constructed in lambda gt10. Sequence analysis of the clone predicted an amino acid sequence in agreement with a published primary structure. Inspection of the codon usage revealed a strong preference for G or C nucleotides at the third codon position as found for several other skeletal muscle proteins. This cDNA clone should facilitate identification of functional domains, including the calmodulin-binding site, and investigation of the molecular basis of X-linked phosphorylase kinase deficiencies.

摘要

合成寡核苷酸已被用于从λgt10构建的cDNA文库中分离出一个1.85 kb的克隆，该克隆包含兔骨骼肌磷酸化酶激酶催化亚基的全长编码序列。对该克隆的序列分析预测的氨基酸序列与已发表的一级结构一致。对密码子使用情况的检查表明，与其他几种骨骼肌蛋白一样，在密码子的第三个位置强烈偏好G或C核苷酸。这个cDNA克隆应该有助于鉴定功能结构域，包括钙调蛋白结合位点，并有助于研究X连锁的磷酸化酶激酶缺陷的分子基础。

相似文献

1
Isolation and sequence analysis of a cDNA clone encoding the entire catalytic subunit of phosphorylase kinase.编码磷酸化酶激酶完整催化亚基的cDNA克隆的分离与序列分析
FEBS Lett. 1987 Aug 10;220(1):36-42. doi: 10.1016/0014-5793(87)80871-2.
2
cDNA cloning and complete primary structure of skeletal muscle phosphorylase kinase (alpha subunit).骨骼肌磷酸化酶激酶（α亚基）的cDNA克隆及完整一级结构
Proc Natl Acad Sci U S A. 1988 May;85(9):2929-33. doi: 10.1073/pnas.85.9.2929.
3
The alpha and beta subunits of phosphorylase kinase are homologous: cDNA cloning and primary structure of the beta subunit.磷酸化酶激酶的α亚基和β亚基是同源的：β亚基的cDNA克隆及一级结构
Proc Natl Acad Sci U S A. 1988 Dec;85(24):9381-5. doi: 10.1073/pnas.85.24.9381.
4
Isolation and sequence analysis of a cDNA clone encoding the entire catalytic subunit of a type-2A protein phosphatase.编码2A型蛋白磷酸酶完整催化亚基的cDNA克隆的分离与序列分析。
FEBS Lett. 1987 Sep 14;221(2):415-22. doi: 10.1016/0014-5793(87)80966-3.
5
Isolation of cDNA clones for the catalytic gamma subunit of mouse muscle phosphorylase kinase: expression of mRNA in normal and mutant Phk mice.小鼠肌肉磷酸化酶激酶催化性γ亚基cDNA克隆的分离：正常和突变型Phk小鼠中mRNA的表达
Proc Natl Acad Sci U S A. 1987 May;84(9):2886-90. doi: 10.1073/pnas.84.9.2886.
6
Molecular cloning of a cDNA for the catalytic subunit of rabbit muscle phosphorylase phosphatase.
FASEB J. 1988 Nov;2(14):3010-6. doi: 10.1096/fasebj.2.14.2846396.
7
Human cDNA encoding the muscle isoform of the phosphorylase kinase gamma subunit (PHKG1).编码磷酸化酶激酶γ亚基（PHKG1）肌肉同工型的人互补DNA。
Hum Genet. 1995 Nov;96(5):616-8. doi: 10.1007/BF00197422.
8
A second catalytic subunit of type-2A protein phosphatase from rabbit skeletal muscle.来自兔骨骼肌的2A型蛋白磷酸酶的第二个催化亚基。
FEBS Lett. 1987 Dec 21;226(1):176-8. doi: 10.1016/0014-5793(87)80574-4.
9
Isolation and sequence analysis of a cDNA clone encoding a type-1 protein phosphatase catalytic subunit: homology with protein phosphatase 2A.编码1型蛋白磷酸酶催化亚基的cDNA克隆的分离与序列分析：与蛋白磷酸酶2A的同源性
FEBS Lett. 1987 Nov 2;223(2):340-6. doi: 10.1016/0014-5793(87)80316-2.
10
Isolation of cDNA encoding the human liver phosphorylase kinase alpha subunit (PHKA2) and identification of a missense mutation of the PHKA2 gene in a family with liver phosphorylase kinase deficiency.编码人肝脏磷酸化酶激酶α亚基（PHKA2）的cDNA的分离及在一个肝脏磷酸化酶激酶缺乏家族中PHKA2基因错义突变的鉴定。
Biochem Mol Biol Int. 1995 Jul;36(3):505-11.

引用本文的文献

1
The association of phosphorylase kinase with membranes of rat liver smooth endoplasmic reticulum.磷酸化酶激酶与大鼠肝脏滑面内质网膜的关联。
Mol Cell Biochem. 1996 Jan 26;154(2):153-63. doi: 10.1007/BF00226783.
2
The substrate specificity and structure of mitogen-activated protein (MAP) kinase-activated protein kinase-2.丝裂原活化蛋白（MAP）激酶激活的蛋白激酶-2的底物特异性与结构
Biochem J. 1993 Dec 15;296 ( Pt 3)(Pt 3):843-9. doi: 10.1042/bj2960843.
3
RPK1, an essential yeast protein kinase involved in the regulation of the onset of mitosis, shows homology to mammalian dual-specificity kinases.
RPK1是一种参与有丝分裂起始调控的必需酵母蛋白激酶，与哺乳动物双特异性激酶具有同源性。
Mol Gen Genet. 1994 Jun 15;243(6):641-53. doi: 10.1007/BF00279573.
4
DPhK-gamma, a putative Drosophila kinase with homology to vertebrate phosphorylase kinase gamma subunits: molecular characterisation of the gene and phenotypic analysis of loss of function mutants.DPhK-γ，一种与脊椎动物磷酸化酶激酶γ亚基具有同源性的假定果蝇激酶：该基因的分子特征及功能缺失突变体的表型分析。
Mol Gen Genet. 1994 Dec 1;245(5):588-97. doi: 10.1007/BF00282221.
5
cDNA cloning and complete primary structure of skeletal muscle phosphorylase kinase (alpha subunit).骨骼肌磷酸化酶激酶（α亚基）的cDNA克隆及完整一级结构
Proc Natl Acad Sci U S A. 1988 May;85(9):2929-33. doi: 10.1073/pnas.85.9.2929.
6
Nucleotide sequence of cDNA encoding the catalytic subunit of phosphorylase kinase from rat soleus muscle.编码大鼠比目鱼肌磷酸化酶激酶催化亚基的cDNA的核苷酸序列。
Nucleic Acids Res. 1988 Mar 25;16(5):2355-6. doi: 10.1093/nar/16.5.2355.
7
Use of DNA sequence and mutant analyses and antisense oligodeoxynucleotides to examine the molecular basis of nonmuscle myosin light chain kinase autoinhibition, calmodulin recognition, and activity.利用DNA序列分析、突变分析和反义寡脱氧核苷酸来研究非肌肉肌球蛋白轻链激酶自身抑制、钙调蛋白识别及活性的分子基础。
J Cell Biol. 1990 Sep;111(3):1107-25. doi: 10.1083/jcb.111.3.1107.
8
Myopathic evolution of an exertional muscle pain syndrome with phosphorylase b kinase deficiency.伴有磷酸化酶b激酶缺乏的劳力性肌肉疼痛综合征的肌病性演变。
Acta Neuropathol. 1990;81(1):84-8. doi: 10.1007/BF00662642.
9
Molecular genetics of phosphorylase kinase: cDNA cloning, chromosomal mapping and isoform structure.磷酸化酶激酶的分子遗传学：cDNA克隆、染色体定位及同工型结构
J Inherit Metab Dis. 1990;13(4):435-41. doi: 10.1007/BF01799500.
10
Complete coding sequences of cDNAs of four variants of rabbit skeletal muscle troponin T.兔骨骼肌肌钙蛋白T四种变体的cDNA完整编码序列。
J Muscle Res Cell Motil. 1991 Dec;12(6):560-5. doi: 10.1007/BF01738444.