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小鼠肌肉磷酸化酶激酶催化性γ亚基cDNA克隆的分离：正常和突变型Phk小鼠中mRNA的表达

Isolation of cDNA clones for the catalytic gamma subunit of mouse muscle phosphorylase kinase: expression of mRNA in normal and mutant Phk mice.

作者信息

Chamberlain J S, VanTuinen P, Reeves A A, Philip B A, Caskey C T

出版信息

Proc Natl Acad Sci U S A. 1987 May;84(9):2886-90. doi: 10.1073/pnas.84.9.2886.

DOI:10.1073/pnas.84.9.2886
PMID:3472241
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC304765/
Abstract

We have isolated and characterized cDNA clones for the gamma subunit of mouse muscle phosphorylase kinase (gamma-Phk). These clones were isolated from a lambda gt11 mouse muscle cDNA library via screening with a synthetic oligonucleotide probe corresponding to a portion of the rabbit gamma-Phk amino acid sequence. The gamma-Phk cDNA clones code for a 387-amino acid protein that shares 93% amino acid sequence identity with the corresponding rabbit amino acid sequence. RNA gel blot analysis reveals that the muscle gamma-Phk probe hybridizes to two mRNA species (2.4 and 1.6 kilobases) in skeletal muscle, cardiac muscle, and brain, but does not hybridize to liver RNA. Phk-deficient I-strain (Phk) mouse muscle contains reduced levels of gamma-Phk mRNA as compared with control mice. Although the Phk defect is an X-linked recessive trait, hybridization to a human-rodent somatic cell hybrid mapping panel shows that the gamma-Phk gene is not located on the X chromosome. Rather, the gamma-Phk cross-hybridizing human restriction fragments map to human chromosomes 7 (multiple) and 11 (single). Reduced gamma-Phk mRNA in I-strain mice, therefore, appears to be a consequence of the Phk-mutant trait and does not stem from a mutant gamma-subunit gene.

摘要

我们已经分离并鉴定了小鼠肌肉磷酸化酶激酶（γ-Phk）γ亚基的cDNA克隆。这些克隆是通过用与兔γ-Phk氨基酸序列一部分相对应的合成寡核苷酸探针筛选λgt11小鼠肌肉cDNA文库而分离得到的。γ-Phk cDNA克隆编码一种387个氨基酸的蛋白质，该蛋白质与相应的兔氨基酸序列具有93%的氨基酸序列同一性。RNA凝胶印迹分析表明，肌肉γ-Phk探针在骨骼肌、心肌和脑中与两种mRNA种类（2.4和1.6千碱基）杂交，但不与肝脏RNA杂交。与对照小鼠相比，Phk缺陷的I品系（Phk）小鼠肌肉中γ-Phk mRNA水平降低。尽管Phk缺陷是一种X连锁隐性性状，但与人类-啮齿动物体细胞杂交定位板杂交显示，γ-Phk基因不在X染色体上。相反，γ-Phk交叉杂交的人类限制性片段定位于人类染色体7（多个）和11（单个）。因此，I品系小鼠中γ-Phk mRNA的减少似乎是Phk突变性状的结果，而不是源于突变的γ亚基基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8e1/304765/d2afe859a062/pnas00274-0334-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8e1/304765/5054dd9b4b8a/pnas00274-0332-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8e1/304765/38000fb140db/pnas00274-0332-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8e1/304765/663a57689aef/pnas00274-0333-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8e1/304765/8a035d33e92e/pnas00274-0333-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8e1/304765/b2b5a20cdbf6/pnas00274-0334-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8e1/304765/f41f8c62e189/pnas00274-0334-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8e1/304765/d2afe859a062/pnas00274-0334-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8e1/304765/5054dd9b4b8a/pnas00274-0332-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8e1/304765/38000fb140db/pnas00274-0332-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8e1/304765/663a57689aef/pnas00274-0333-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8e1/304765/8a035d33e92e/pnas00274-0333-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8e1/304765/b2b5a20cdbf6/pnas00274-0334-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8e1/304765/f41f8c62e189/pnas00274-0334-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8e1/304765/d2afe859a062/pnas00274-0334-c.jpg

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引用本文的文献

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本文引用的文献

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THE ONTOGENY OF CREATINE KINASE ISOZYMES.肌酸激酶同工酶的个体发生
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The relation of phosphorylase to glycogenolysis in skeletal muscle and heart of mice.小鼠骨骼肌和心脏中磷酸化酶与糖原分解的关系。
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Hum Genet. 1995 Nov;96(5):616-8. doi: 10.1007/BF00197422.
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The gamma phosphorylase kinase gene, Phkg, maps to mouse chromosome 5 near Gus.γ磷酸化酶激酶基因Phkg定位于小鼠5号染色体上靠近Gus的位置。
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DPhK-gamma, a putative Drosophila kinase with homology to vertebrate phosphorylase kinase gamma subunits: molecular characterisation of the gene and phenotypic analysis of loss of function mutants.DPhK-γ，一种与脊椎动物磷酸化酶激酶γ亚基具有同源性的假定果蝇激酶：该基因的分子特征及功能缺失突变体的表型分析。
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Nucleotide sequence of cDNA encoding the catalytic subunit of phosphorylase kinase from rat soleus muscle.编码大鼠比目鱼肌磷酸化酶激酶催化亚基的cDNA的核苷酸序列。
Nucleic Acids Res. 1988 Mar 25;16(5):2355-6. doi: 10.1093/nar/16.5.2355.
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Expression of human and Chinese hamster hypoxanthine-guanine phosphoribosyltransferase cDNA recombinants in cultured Lesch-Nyhan and Chinese hamster fibroblasts.人和中国仓鼠次黄嘌呤-鸟嘌呤磷酸核糖基转移酶cDNA重组体在培养的莱施-尼汉病患者及中国仓鼠成纤维细胞中的表达
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A practical approach for quantitating specific mRNAs by solution hybridization.一种通过溶液杂交对特定信使核糖核酸进行定量的实用方法。
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