Coderre T J, Wall P D
Department of Anatomy and Embryology, University College London, England, U.K.
Pharmacol Biochem Behav. 1988 Mar;29(3):461-6. doi: 10.1016/0091-3057(88)90004-4.
Arthritis was induced in ether anesthetised rats by injecting 1.25 mg of sodium urate crystals into the ankle joint. Twenty-four hr after the injection the ankle is swollen and the animal does not place full weight on the affected foot. The ankle is more sensitive than normal to movement and pressure. Responses to stimulation of the foot and toes on the arthritic limb are reduced due to a reluctance to move the affected limb. These measures, which reflect ongoing pain, hyperalgesia or tenderness and guarding, are attenuated in animals treated with dexamethasone, phenylbutazone, and morphine, as well as in animals whose nerves to the ankle had been pretreated with capsaicin. Guanethidine and colchicine failed to influence the behavioural responses to the urate injection. Ankle joint urate arthritis has advantages over other models of arthritis for therapeutic testing in that in a short time it affects a single joint in rats, and it produces responses which can be assessed by simple, sensitive measures.
通过向乙醚麻醉的大鼠踝关节注射1.25毫克尿酸钠晶体来诱导关节炎。注射后24小时，踝关节肿胀，动物患足无法完全负重。踝关节对运动和压力比正常情况更敏感。由于不愿移动患侧肢体，对关节炎肢体的足部和脚趾刺激的反应会降低。这些反映持续疼痛、痛觉过敏或压痛以及保护性反应的指标，在接受地塞米松、保泰松和吗啡治疗的动物以及踝关节神经预先用辣椒素处理的动物中会减弱。胍乙啶和秋水仙碱未能影响对尿酸注射的行为反应。踝关节尿酸盐关节炎在治疗测试方面比其他关节炎模型更具优势，因为在短时间内它会影响大鼠的单个关节，并且会产生可以通过简单、灵敏的指标进行评估的反应。
